Archives of Clinical Infectious Diseases

Published by: Kowsar

Detection of Brucella spp. in Dairy Products by Real-Time PCR

Elham Moslemi 1 , Mohammad Mehdi Soltandalal 2 , 3 , Mohammad Reza Beheshtizadeh 1 , Afsoon Taghavi 4 , Hamidreza Kheiri Manjili 5 , Reza Mahmoudi Lamouki 6 and Amir Izadi 7 , 8 , *
Authors Information
1 Department of Biology, East Tehran Branch, Islamic Azad University, Tehran, Iran
2 Food Microbiology Research Center, Tehran University of Medical Sciences, Tehran, Iran
3 Division of Microbiology, Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
4 Research Cancer Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
5 Assistant Professor, Department of Pharmaceutical Nanotechnology, School of Pharmacy, Zanjan University of Medical Sciences, Zanjan, Iran
6 Department of Medical Genetic, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
7 Banej Exir Research Center, Tehran, Iran
8 Young Researcher Club, East Tehran Branch, Islamic Azad University, Tehran, Iran
Article information
  • Archives of Clinical Infectious Diseases: January 2018, 13 (1); e12673
  • Published Online: January 27, 2018
  • Article Type: Research Article
  • Received: October 17, 2016
  • Revised: May 6, 2017
  • Accepted: September 18, 2017
  • DOI: 10.5812/archcid.12673

To Cite: Moslemi E, Soltandalal M M, Beheshtizadeh M R, Taghavi A, Kheiri Manjili H, et al. Detection of Brucella spp. in Dairy Products by Real-Time PCR, Arch Clin Infect Dis. 2018 ; 13(1):e12673. doi: 10.5812/archcid.12673.

Copyright © 2018, Archives of Clinical Infectious Diseases. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License ( which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited
1. Background
2. Methods
3. Results
4. Discussion
  • 1. Committee on Infectious D, Committee on N, American Academy of P. Consumption of raw or unpasteurized milk and milk products by pregnant women and children. Pediatrics. 2014;133(1):175-9. doi: 10.1542/peds.2013-3502. [PubMed: 24344105].
  • 2. Wareth G, Melzer F, Elschner MC, Neubauer H, Roesler U. Detection of Brucella melitensis in bovine milk and milk products from apparently healthy animals in Egypt by real-time PCR. J Infect Dev Ctries. 2014;8(10):1339-43. doi: 10.3855/jidc.4847. [PubMed: 25313613].
  • 3. Yaran M, Najafi S, Shoaei P, Ataei B, Fadaei R, Ramazanpour J, et al. Prevalence of Brucella melitensis and Brucella abortus in raw milk and dairy product by real time PCR technique. Ulutas Med J. 2016;2(1):7-11. doi: 10.5455/umj.20151004054235.
  • 4. Ilhan Z, Solmaz H, Aksakal A, Gulhan T, Ekin IH, Boynukara B. Detection of Brucella melitensis DNA in the milk of sheep after abortion by PCR assay. Arch Med Vet. 2008;40(2):141-6. doi: 10.4067/s0301-732x2008000200005.
  • 5. Tay BY, Ahmad N, Hashim R, Mohamed Zahidi J, Thong KL, Koh XP, et al. Multiple-locus variable-number tandem-repeat analysis (MLVA) genotyping of human Brucella isolates in Malaysia. BMC Infect Dis. 2015;15:220. doi: 10.1186/s12879-015-0958-0. [PubMed: 26033227].
  • 6. Celebi O, Celebi D, Eda Balkan C. Effects of boiling dairy products on human brucellosis. Eurasian J Med. 2013;45(2):73-6. doi: 10.5152/eajm.2013.17. [PubMed: 25610256].
  • 7. Wang Z, Bie P, Cheng J, Wu Q, Lu L. In vitro evaluation of six chemical agents on smooth Brucella melitensis strain. Ann Clin Microbiol Antimicrob. 2015;14:16. doi: 10.1186/s12941-015-0077-1. [PubMed: 25857255].
  • 8. Sanodze L, Bautista CT, Garuchava N, Chubinidze S, Tsertsvadze E, Broladze M, et al. Expansion of brucellosis detection in the country of Georgia by screening household members of cases and neighboring community members. BMC Public Health. 2015;15:459. doi: 10.1186/s12889-015-1761-y. [PubMed: 25934639].
  • 9. Osoro EM, Munyua P, Omulo S, Ogola E, Ade F, Mbatha P, et al. Strong Association Between Human and Animal Brucella Seropositivity in a Linked Study in Kenya, 2012-2013. Am J Trop Med Hyg. 2015;93(2):224-31. doi: 10.4269/ajtmh.15-0113. [PubMed: 26101275].
  • 10. Franz DR, Jahrling PB, Friedlander AM, McClain DJ, Hoover DL, Bryne WR, et al. Clinical recognition and management of patients exposed to biological warfare agents. JAMA. 1997;278(5):399-411. doi: 10.1001/jama.278.5.399. [PubMed: 9244332].
  • 11. Colmenero JD, Reguera JM, Martos F, Sanchez-De-Mora D, Delgado M, Causse M, et al. Complications associated with Brucella melitensis infection: a study of 530 cases. Medicine (Baltimore). 1996;75(4):195-211. doi: 10.1097/00005792-199607000-00003. [PubMed: 8699960].
  • 12. Izadi A, Moslemi E, Tabatabaei Panah AS, Kheiri Manjili H. Brucella Spp. detection in dairy products using Nested and Hemi Nested PCR techniques. Ann Biol Res. 2014;5(1):124-31.
  • 13. Baily GG, Krahn JB, Drasar BS, Stoker NG. Detection of Brucella melitensis and Brucella abortus by DNA amplification. J Trop Med Hyg. 1992;95(4):271-5. [PubMed: 1495123].
  • 14. Bricker BJ. PCR as a diagnostic tool for brucellosis. Vet Microbiol. 2002;90(1-4):435-46. doi: 10.1016/S0378-1135(02)00228-6. [PubMed: 12414163].
  • 15. Kami M, Fukui T, Ogawa S, Kazuyama Y, Machida U, Tanaka Y, et al. Use of real-time PCR on blood samples for diagnosis of invasive aspergillosis. Clin Infect Dis. 2001;33(9):1504-12. doi: 10.1086/323337. [PubMed: 11588697].
  • 16. Hamdy ME, Amin AS. Detection of Brucella species in the milk of infected cattle, sheep, goats and camels by PCR. Vet J. 2002;163(3):299-305. doi: 10.1053/tvjl.2001.0681. [PubMed: 12090772].
  • 17. Ohtsuki R, Kawamoto K, Kato Y, Shah MM, Ezaki T, Makino SI. Rapid detection of Brucella spp. by the loop-mediated isothermal amplification method. J Appl Microbiol. 2008;104(6):1815-23. doi: 10.1111/j.1365-2672.2008.03732.x. [PubMed: 18248366].
  • 18. Sanaei Dashti A, Karimi A, Javad V, Shiva F, Fallah F, Alaei MR, et al. ELISA Cut-off Point for the Diagnosis of Human Brucellosis; a Comparison with Serum Agglutination Test. Iran J Med Sci. 2012;37(1):9-14. [PubMed: 23115425].
  • 19. Atluri VL, Xavier MN, de Jong MF, den Hartigh AB, Tsolis RM. Interactions of the human pathogenic Brucella species with their hosts. Annu Rev Microbiol. 2011;65:523-41. doi: 10.1146/annurev-micro-090110-102905. [PubMed: 21939378].
  • 20. Rasmussen R. In Rapid cycle real-time PCR. Quantification on the LightCycler. Springer Berlin Heidelberg; 2001. p. 21-34.
  • 21. Capparelli R, Parlato M, Iannaccone M, Roperto S, Marabelli R, Roperto F, et al. Heterogeneous shedding of Brucella abortus in milk and its effect on the control of animal brucellosis. J Appl Microbiol. 2009;106(6):2041-7. doi: 10.1111/j.1365-2672.2009.04177.x. [PubMed: 19298512].
  • 22. Memish Z, Mah MW, Al Mahmoud S, Al Shaalan M, Khan MY. Brucella bacteraemia: clinical and laboratory observations in 160 patients. J Infect. 2000;40(1):59-63. doi: 10.1053/jinf.1999.0586. [PubMed: 10762113].
  • 23. Pappas G, Papadimitriou P, Akritidis N, Christou L, Tsianos EV. The new global map of human brucellosis. Lancet Infect Dis. 2006;6(2):91-9. doi: 10.1016/S1473-3099(06)70382-6. [PubMed: 16439329].
  • 24. Cutler SJ, Whatmore AM, Commander NJ. Brucellosis--new aspects of an old disease. J Appl Microbiol. 2005;98(6):1270-81. doi: 10.1111/j.1365-2672.2005.02622.x. [PubMed: 15916641].
  • 25. Gwida M, Al Dahouk S, Melzer F, Rosler U, Neubauer H, Tomaso H. Brucellosis - regionally emerging zoonotic disease? Croat Med J. 2010;51(4):289-95. doi: 10.3325/cmj.2010.51.289. [PubMed: 20718081].
  • 26. Moradi G, Nasab NE, Ghaderi E, Sofi Majidpour M, Hamideh Salimzadeh. H . Brucellosis in Kurdistan Province from 1997 to 2003. Ann Alquds Med. 2006;2(1):32-7.
  • 27. Yu WL, Nielsen K. Review of detection of Brucella spp. by polymerase chain reaction. Croat Med J. 2010;51(4):306-13. [PubMed: 20718083].
  • 28. Garshasbi M, Ramazani A, Sorouri R, Javani S, Moradi S. Molecular detection of Brucella species in patients suspicious of Brucellosis from Zanjan, Iran. Braz J Microbiol. 2014;45(2):533-8. [PubMed: 25242938].
  • 29. Guarino A, Serpe L, Fusco G, Scaramuzzo A, Gallo P. Detection of Brucella species in buffalo whole blood by gene-specific PCR. Vet Rec. 2000;147(22):634-6. doi: 10.1136/vr.147.22.634. [PubMed: 11128080].
  • 30. Lindahl-Rajala E, Hoffman T, Fretin D, Godfroid J, Sattorov N, Boqvist S, et al. Detection and characterization of Brucella spp. in bovine milk in small-scale urban and peri-urban farming in Tajikistan. PLoS Negl Trop Dis. 2017;11(3). e0005367. doi: 10.1371/journal.pntd.0005367. [PubMed: 28296882].
  • 31. Probert WS, Schrader KN, Khuong NY, Bystrom SL, Graves MH. Real-time multiplex PCR assay for detection of Brucella spp., B. abortus, and B. melitensis. J Clin Microbiol. 2004;42(3):1290-3. doi: 10.1128/JCM.42.3.1290-1293.2004. [PubMed: 15004098].
  • 32. Rodríguez-Lázaro D, López-Enríquez L, Ocampo-Sosa AA, Muñoz P, Blasco JM, Marín C, et al. Evaluation of eryC as a molecular marker for the quantitative detection of Brucella Spp. by real-time PCR in food samples. Food Anal Methods. 2017;10(5):1148-55. doi: 10.1007/s12161-017-0822-5.
  • 33. Kaden R, Ferrari S, Alm E, Wahab T. A novel real-time PCR assay for specific detection of Brucella melitensis. BMC Infect Dis. 2017;17(1):230. doi: 10.1186/s12879-017-2327-7. [PubMed: 28340558].

Featured Image:

Creative Commons License Except where otherwise noted, this work is licensed under Creative Commons Attribution Non Commercial 4.0 International License .

Search Relations:



Create Citiation Alert
via Google Reader

Readers' Comments