Archives of Clinical Infectious Diseases

Published by: Kowsar

Evaluation of Recombinant SRS3 Antigen for Diagnosis of Toxoplasmosis by Enzyme-Linked Immunosorbent Assay

Seyedeh Mahya Marashiyan 1 , Fatemeh Moradian 1 , Geita Saadatnia 2 , * and Majid Golkar 3
Authors Information
1 Department of Basic Sciences, Sari Agricultural Sciences and Natural Resources University, Sari, Iran
2 Department of Biotechnology, Iranian Research Organization for Science and Technology (IROST), Tehran, Iran
3 Molecular Parasitology Laboratory, Department of Parasitology, Pasteur Institute of Iran, Tehran, Iran
Article information
  • Archives of Clinical Infectious Diseases: January 2017, 12 (1); e35612
  • Published Online: November 15, 2016
  • Article Type: Research Article
  • Received: December 19, 2015
  • Revised: October 31, 2016
  • Accepted: November 6, 2016
  • DOI: 10.5812/archcid.35612

To Cite: Marashiyan S M, Moradian F, Saadatnia G, Golkar M. Evaluation of Recombinant SRS3 Antigen for Diagnosis of Toxoplasmosis by Enzyme-Linked Immunosorbent Assay, Arch Clin Infect Dis. 2017 ; 12(1):e35612. doi: 10.5812/archcid.35612.

Copyright © 2016, Infectious Diseases and Tropical Medicine Research Center. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License ( which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
1. Background
2. Objectives
3. Methods
4. Results
5. Discussion
  • 1. Yap GS, Sher A. Cell-mediated immunity to Toxoplasma gondii: initiation, regulation and effector function. Immunobiology. 1999; 201(2): 240-7[DOI][PubMed]
  • 2. Boothroyd JC, Hehl A, Knoll LJ, Manger ID. The surface of Toxoplasma: more and less. Int J Parasitol. 1998; 28(1): 3-9[DOI][PubMed]
  • 3. Darcy F, Santoro F. Parasitic infections and the immune system. 1994; : 163-20
  • 4. Sims T, Hay J, Talbot I. An electron microscope and immunohistochemical study of the intracellular location of Toxoplasma tissue cysts within the brains of mice with congenital toxoplasmosis. British J Experiment Pathol. 1989; 90(3): 317-25
  • 5. Lekutis C, Ferguson DJ, Grigg ME, Camps M, Boothroyd JC. Surface antigens of Toxoplasma gondii: variations on a theme. Int J Parasitol. 2001; 31(12): 1285-92[PubMed]
  • 6. Dzierszinski F, Mortuaire M, Cesbron-Delauw MF, Tomavo S. Targeted disruption of the glycosylphosphatidylinositol-anchored surface antigen SAG3 gene in Toxoplasma gondii decreases host cell adhesion and drastically reduces virulence in mice. Mol Microbiol. 2000; 37(3): 574-82[DOI][PubMed]
  • 7. Jung C, Lee CY, Grigg ME. The SRS superfamily of Toxoplasma surface proteins. Int J Parasitol. 2004; 34(3): 285-96[DOI][PubMed]
  • 8. Rollins SM, Peppercorn A, Young JS, Drysdale M, Baresch A, Bikowski MV, et al. Application of in vivo induced antigen technology (IVIAT) to Bacillus anthracis. PLoS One. 2008; 3(3): 1824[DOI][PubMed]
  • 9. Amerizadeh A, Idris ZM, Khoo BY, Kotresha D, Yunus MH, Karim IZ, et al. Identification of Toxoplasma gondii in-vivo induced antigens by cDNA library immunoscreening with chronic toxoplasmosis sera. Microb Pathog. 2013; 54: 60-6[DOI][PubMed]
  • 10. Gross U, Holpert M, Goebel S. Impact of stage differentiation on diagnosis of toxoplasmosis. Ann Ist Super Sanita. 2004; 40(1): 65-70[PubMed]
  • 11. Gatkowska J, Hiszczynska-Sawicka E, Kur J, Holec L, Dlugonska H. Toxoplasma gondii: an evaluation of diagnostic value of recombinant antigens in a murine model. Exp Parasitol. 2006; 114(3): 220-7[DOI][PubMed]
  • 12. Piergili Fioretti D. [Problems and limitations of conventional and innovative methods for the diagnosis of Toxoplasmosis in humans and animals]. Parassitologia. 2004; 46(1-2): 177-81[PubMed]
  • 13. Pietkiewicz H, Hiszczynska-Sawicka E, Kur J, Petersen E, Nielsen HV, Paul M, et al. Usefulness of Toxoplasma gondii recombinant antigens (GRA1, GRA7 and SAG1) in an immunoglobulin G avidity test for the serodiagnosis of toxoplasmosis. Parasitol Res. 2007; 100(2): 333-7[DOI][PubMed]
  • 14. Suzuki LA, Rocha RJ, Rossi CL. Evaluation of serological markers for the immunodiagnosis of acute acquired toxoplasmosis. J Med Microbiol. 2001; 50(1): 62-70[DOI][PubMed]
  • 15. Pietkiewicz H, Hiszczyriska-Sawicka E, Kur J, Petersen E, Nielsen HV, Stankiewicz M, et al. Usefulness of Toxoplasma gonidi specific recombinant antigens in serodiagnosis of human toxoplamosis. Clin Diagnostic laboratory Immunol. 2004; 42(4): 1779-81
  • 16. Fallahi S, Seyyed Tabaei SJ, Pournia Y, Zebardast N, Kazemi B. Comparison of loop-mediated isothermal amplification (LAMP) and nested-PCR assay targeting the RE and B1 gene for detection of Toxoplasma gondii in blood samples of children with leukaemia. Diagn Microbiol Infect Dis. 2014; 79(3): 347-54[DOI][PubMed]
  • 17. Fallahi S, Arab Mazar Z, Ghasemian M, Haghighi A. Challenging loop-mediated isothermal amplification (LAMP) technique for molecular detection of Toxoplasma gondii. Asian Pacific J Tropical Med. 2015; 3: 366-72[DOI]
  • 18. Manger ID, Hehl AB, Boothroyd JC. The surface of Toxoplasma tachyzoites is dominated by a family of glycosylphosphatidylinositol-anchored antigens related to SAG1. Infect Immun. 1998; 66(5): 2237-44[PubMed]
  • 19. Buffolano W, Beghetto E, Del Pezzo M, Spadoni A, Di Cristina M, Petersen E, et al. Use of recombinant antigens for early postnatal diagnosis of congenital toxoplasmosis. J Clin Microbiol. 2005; 43(12): 5916-24[DOI][PubMed]
  • 20. Handman E, Remington JS. Antibody responses to toxoplasma antigens in mice infected with strains of different virulence. Infect Immun. 1980; 29(1): 215-20[PubMed]
  • 21. Allahyari M, Mohabati R, Babaie J, Amiri S, Siavashani ZJ, Zare M, et al. Production of in-vitro refolded and highly antigenic SAG1 for development of a sensitive and specific Toxoplasma IgG ELISA. J Immunol Methods. 2015; 416: 157-66[DOI][PubMed]
  • 22. Khanaliha K, Motazedian MH, Kazemi B, Shahriari B, Bandehpour M, Sharifniya Z. Evaluation of recombinant SAG1, SAG2, and SAG3 antigens for serodiagnosis of toxoplasmosis. Korean J Parasitol. 2014; 52(2): 137-42[DOI][PubMed]
  • 23. Jalallou N, Bandepour M, Khazan H, Haghighi A, Abdollahi S, Kazemi B. Recombinant SAG1 Antigen to Detect Toxoplasma gondii Specific Immunoglobulin G in Human Sera by ELISA Test. Iran J Parasitol. 2010; 5(2): 1-9[PubMed]
  • 24. Selseleh MM, Keshavarz H, Mohebali M, Shojaee S, Modarressi M, Eshragian M, et al. Production and Evaluation of Toxoplasma gondii Recombinant Surface Antigen 1 (SAG1) for Serodiagnosis of Acute and Chronic Toxoplasma Infection in Human Sera. Iran J Parasitol. 2012; 7(3): 1-9[PubMed]
Creative Commons License Except where otherwise noted, this work is licensed under Creative Commons Attribution Non Commercial 4.0 International License .

Search Relations:



Create Citiation Alert
via Google Reader

Readers' Comments