Rapid and Specific Polymerase Chain Reaction-Enzyme Linked Immunosorbent Assay for Detection of Escherichia coli LT Toxin From Clinical Isolates

Authors Information
Article Notes and Dates
To Cite : Esfandiari P, Amani J, Imani Fouladi A A, Nazarian S, Mirhosseini A, et al. Rapid and Specific Polymerase Chain Reaction-Enzyme Linked Immunosorbent Assay for Detection of Escherichia coli LT Toxin From Clinical Isolates, Arch Clin Infect Dis. 2017 ;12(1):e36261. doi: 10.5812/archcid.36261.
Abstract
1. Background
2. Objectives
3. Methods
4. Results
5. Discussion
Footnote
References
  • 1. Nazarian S, Gargari SL, Rasooli I, Hasannia S, Pirooznia N. A PLGA-encapsulated chimeric protein protects against adherence and toxicity of enterotoxigenic Escherichia coli. Microbiol Res. 2014; 169(2-3): 205-12[DOI][PubMed]
  • 2. Zhang W, Sack DA. Current Progress in Developing Subunit Vaccines against Enterotoxigenic Escherichia coli-Associated Diarrhea. Clin Vaccine Immunol. 2015; 22(9): 983-91[DOI][PubMed]
  • 3. Hill DR, Beeching NJ. Travelers' diarrhea. Curr Opin Infect Dis. 2010; 23(5): 481-7[DOI][PubMed]
  • 4. Shah N, DuPont HL, Ramsey DJ. Global etiology of travelers' diarrhea: systematic review from 1973 to the present. Am J Trop Med Hyg. 2009; 80(4): 609-14[PubMed]
  • 5. Marchou B. [Traveller's diarrhea: epidemiology, clinical practice guideline for the prevention and treatment]. Presse Med. 2013; 42(1): 76-81[DOI][PubMed]
  • 6. Walker RI, Steele D, Aguado T, Ad Hoc ETEC. Analysis of strategies to successfully vaccinate infants in developing countries against enterotoxigenic E. coli (ETEC) disease. Vaccine. 2007; 25(14): 2545-66[DOI][PubMed]
  • 7. Kolenda R, Burdukiewicz M, Schierack P. A systematic review and meta-analysis of the epidemiology of pathogenic Escherichia coli of calves and the role of calves as reservoirs for human pathogenic E. coli. Front Cell Infect Microbiol. 2015; 5: 23[DOI][PubMed]
  • 8. Madhavan TP, Sakellaris H. Colonization factors of enterotoxigenic Escherichia coli. Adv Appl Microbiol. 2015; 90: 155-97[DOI][PubMed]
  • 9. Sjoling A, von Mentzer A, Svennerholm AM. Implications of enterotoxigenic Escherichia coli genomics for vaccine development. Expert Rev Vaccines. 2015; 14(4): 551-60[DOI][PubMed]
  • 10. Fleckenstein J, Sheikh A, Qadri F. Novel antigens for enterotoxigenic Escherichia coli vaccines. Expert Rev Vaccines. 2014; 13(5): 631-9[DOI][PubMed]
  • 11. Munson GP. Virulence regulons of enterotoxigenic Escherichia coli. Immunol Res. 2013; 57(1-3): 229-36[DOI][PubMed]
  • 12. Giannella RA. Suckling mouse model for detection of heat-stable Escherichia coli enterotoxin: characteristics of the model. Infect Immun. 1976; 14(1): 95-9[PubMed]
  • 13. Giannella RA, Drake KW, Luttrell M. Development of a radioimmunoassay for Escherichia coli heat-stable enterotoxin: comparison with the suckling mouse bioassay. Infect Immun. 1981; 33(1): 186-92[PubMed]
  • 14. Cryan B. Comparison of three assay systems for detection of enterotoxigenic Escherichia coli heat-stable enterotoxin. J Clin Microbiol. 1990; 28(4): 792-4[PubMed]
  • 15. Antikainen J, Kantele A, Pakkanen SH, Laaveri T, Riutta J, Vaara M, et al. A quantitative polymerase chain reaction assay for rapid detection of 9 pathogens directly from stools of travelers with diarrhea. Clin Gastroenterol Hepatol. 2013; 11(10): 1300-7[DOI][PubMed]
  • 16. Nazarian S, Gargari SL, Rasooli I, Alerasol M, Bagheri S, Alipoor SD. Prevalent phenotypic and genotypic profile of enterotoxigenic Escherichia coli among Iranian children. Jpn J Infect Dis. 2014; 67(2): 78-85[PubMed]
  • 17. Wilson K. Preparation of genomic DNA from bacteria. 2001;
  • 18. Bagheri S, Mousavi Gargari SL, Rasooli I, Nazarian S, Alerasol M. A CssA, CssB and LTB chimeric protein induces protection against Enterotoxigenic Escherichia coli. Braz J Infect Dis. 2014; 18(3): 308-14[DOI][PubMed]
  • 19. Gupta SK, Keck J, Ram PK, Crump JA, Miller MA, Mintz ED. Part III. Analysis of data gaps pertaining to enterotoxigenic Escherichia coli infections in low and medium human development index countries, 1984-2005. Epidemiol Infect. 2008; 136(6): 721-38[DOI][PubMed]
  • 20. Sjoling A, Wiklund G, Savarino SJ, Cohen DI, Svennerholm AM. Comparative analyses of phenotypic and genotypic methods for detection of enterotoxigenic Escherichia coli toxins and colonization factors. J Clin Microbiol. 2007; 45(10): 3295-301[DOI][PubMed]
  • 21. Svennerholm AM, Wiklund G. Rapid GM1-enzyme-linked immunosorbent assay with visual reading for identification of Escherichia coli heat-labile enterotoxin. J Clin Microbiol. 1983; 17(4): 596-600[PubMed]
  • 22. Lothigius A, Janzon A, Begum Y, Sjoling A, Qadri F, Svennerholm AM, et al. Enterotoxigenic Escherichia coli is detectable in water samples from an endemic area by real-time PCR. J Appl Microbiol. 2008; 104(4): 1128-36[DOI][PubMed]
  • 23. Ahmed N, Kamal AM, el-Hamid TA. Comparative study of staphylococcal co-agglutination and latex agglutination for detection of enterotoxigenic Escherichia coli. J Egypt Public Health Assoc. 1993; 68(1-2): 11-9[PubMed]
  • 24. Qadri F, Svennerholm AM, Faruque AS, Sack RB. Enterotoxigenic Escherichia coli in developing countries: epidemiology, microbiology, clinical features, treatment, and prevention. Clin Microbiol Rev. 2005; 18(3): 465-83[DOI][PubMed]
  • 25. Caeiro JP, Estrada-Garcia MT, Jiang ZD, Mathewson JJ, Adachi JA, Steffen R, et al. Improved detection of enterotoxigenic Escherichia coli among patients with travelers' diarrhea, by use of the polymerase chain reaction technique. J Infect Dis. 1999; 180(6): 2053-5[DOI][PubMed]
  • 26. Omar KB, Barnard TG. Detection of diarrhoeagenic Escherichia coli in clinical and environmental water sources in South Africa using single-step 11-gene m-PCR. World J Microbiol Biotechnol. 2014; 30(10): 2663-71[DOI][PubMed]
  • 27. Rajkhowa S, Hussain I, Rajkhowa C. Detection of heat-stable and heat-labile enterotoxin genes of Escherichia coli in diarrhoeic faecal samples of mithun (Bos frontalis) calves by polymerase chain reaction. J Appl Microbiol. 2009; 106(2): 455-8[DOI][PubMed]
  • 28. Blanco M, Blanco JE, Blanco J, Gonzalez EA, Mora A, Prado C, et al. Prevalence and characteristics of Escherichia coli serotype O157:H7 and other verotoxin-producing E. coli in healthy cattle. Epidemiol Infect. 1996; 117(2): 251-7[PubMed]
  • 29. Shahrani M, Dehkordi FS, Momtaz H. Characterization of Escherichia coli virulence genes, pathotypes and antibiotic resistance properties in diarrheic calves in Iran. Biol Res. 2014; 47: 28[DOI][PubMed]
  • 30. Sue MJ, Yeap SK, Omar AR, Tan SW. Application of PCR-ELISA in molecular diagnosis. Biomed Res Int. 2014; 2014: 653014[DOI][PubMed]
  • 31. Kuo JT, Cheng CY, Huang HH, Tsao CF, Chung YC. A rapid method for the detection of representative coliforms in water samples: polymerase chain reaction-enzyme-linked immunosorbent assay (PCR-ELISA). J Ind Microbiol Biotechnol. 2010; 37(3): 237-44[DOI][PubMed]
  • 32. Mousavi SL, Nazarian S, Amani J, Rahgerdi AK. Rapid screening of toxigenic vibrio cholerae O1 strains from south Iran by PCR-ELISA. Iran Biomed J. 2008; 12(1): 15-21[PubMed]
  • 33. Amani J, Ahmadpour A, Imani Fooladi AA, Nazarian S. Detection of E. coli O157:H7 and Shigella dysenteriae toxins in clinical samples by PCR-ELISA. Braz J Infect Dis. 2015; 19(3): 278-84[DOI][PubMed]
Creative Commons License Except where otherwise noted, this work is licensed under Creative Commons Attribution Non Commercial 4.0 International License .

Search Relations:

Author(s):

Article(s):

Create Citiaion Alert via Google Reader

Cited By:

Archives of Clinical Infectious Diseases accepts terms & conditions of:

International Committee of Medical Journal Editors (ICMJE) Citedby Linking DOI enabled Crossref iThenticate COPE Cross Check