Archives of Clinical Infectious Diseases

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Genotyping of Clinical Streptococcus agalactiae Strains Based on Molecular Serotype of Capsular (cps) Gene Cluster Sequences Using Polymerase Chain Reaction

Mohammad Reza Arabestani 1 , 2 , * , Seyed Masoud Mousavi 1 and Mona Nasaj 1
Authors Information
1 Department of Microbiology, Faculty of Medicine, Hamadan University of Medical Sciences, Hamadan, IR Iran
2 Brucellosis Research Center, Hamadan University of Medical Sciences, Hamadan, IR Iran
Article information
  • Archives of Clinical Infectious Diseases: January 2017, 12 (1); e36787
  • Published Online: October 16, 2016
  • Article Type: Research Article
  • Received: January 31, 2016
  • Revised: October 4, 2016
  • Accepted: October 5, 2016
  • DOI: 10.5812/archcid.36787

To Cite: Arabestani M R, Mousavi S M, Nasaj M. Genotyping of Clinical Streptococcus agalactiae Strains Based on Molecular Serotype of Capsular (cps) Gene Cluster Sequences Using Polymerase Chain Reaction, Arch Clin Infect Dis. 2017 ; 12(1):e36787. doi: 10.5812/archcid.36787.

Copyright © 2016, Infectious Diseases and Tropical Medicine Research Center. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License ( which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
1. Background
2. Objectives
3. Methods
4. Results
5. Discussion
  • 1. Verani JR, McGee L, Schrag SJ, Division of Bacterial Diseases NCFI, Respiratory Diseases CFDC. Prevention of perinatal group B streptococcal disease--revised guidelines from CDC, 2010. MMWR Recomm Rep. 2010; 59: 1-36[PubMed]
  • 2. Morozumi M, Wajima T, Takata M, Iwata S, Ubukata K. 1Molecular characteristics of Group B streptococci isolated from adults with invasive infections in Japan. J Clin Microbiol. 2016; [DOI]
  • 3. Gherardi G, Imperi M, Baldassarri L, Pataracchia M, Alfarone G, Recchia S, et al. Molecular epidemiology and distribution of serotypes, surface proteins, and antibiotic resistance among group B streptococci in Italy. J Clin Microbiol. 2007; 45(9): 2909-16[DOI][PubMed]
  • 4. Kong F, Ma L, Gilbert GL. Simultaneous detection and serotype identification of Streptococcus agalactiae using multiplex PCR and reverse line blot hybridization. J Med Microbiol. 2005; 54: 1133-8[DOI][PubMed]
  • 5. Brzychczy-Wloch M, Gosiewski T, Bodaszewska-Lubas M, Adamski P, Heczko PB. Molecular characterization of capsular polysaccharides and surface protein genes in relation to genetic similarity of group B streptococci isolated from Polish pregnant women. Epidemiol Infect. 2012; 140(2): 329-36[DOI][PubMed]
  • 6. Heath PT. An update on vaccination against group B streptococcus. Expert Rev Vaccines. 2011; 10(5): 685-94[DOI][PubMed]
  • 7. Johri AK, Paoletti LC, Glaser P, Dua M, Sharma PK, Grandi G, et al. Group B Streptococcus: global incidence and vaccine development. Nat Rev Microbiol. 2006; 4(12): 932-42[DOI][PubMed]
  • 8. Hickman ME, Rench MA, Ferrieri P, Baker CJ. Changing epidemiology of group B streptococcal colonization. Pediatrics. 1999; 104(2 Pt 1): 203-9[PubMed]
  • 9. van Belkum A, Tassios PT, Dijkshoorn L, Haeggman S, Cookson B, Fry NK, et al. Guidelines for the validation and application of typing methods for use in bacterial epidemiology. Clin Microbiol Infect. 2007; 13 Suppl 3: 1-46[DOI][PubMed]
  • 10. Park CJ, Vandel NM, Ruprai DK, Martin EA, Gates KM, Coker D. Detection of group B streptococcal colonization in pregnant women using direct latex agglutination testing of selective broth. J Clin Microbiol. 2001; 39(1): 408-9[PubMed]
  • 11. Benson JA, Flores AE, Baker CJ, Hillier SL, Ferrieri P. Improved methods for typing nontypeable isolates of group B streptococci. Int J Med Microbiol. 2002; 292(1): 37-42[DOI][PubMed]
  • 12. Zhao Z, Kong F, Gilbert GL. Reverse line blot assay for direct identification of seven Streptococcus agalactiae major surface protein antigen genes. Clin Vaccine Immunol. 2006; 13(1): 145-9[DOI][PubMed]
  • 13. Slotved HC, Kong F, Lambertsen L, Sauer S, Gilbert GL. Serotype IX, a Proposed New Streptococcus agalactiae Serotype. J Clin Microbiol. 2007; 45(9): 2929-36[DOI][PubMed]
  • 14. Imperi M, Pataracchia M, Alfarone G, Baldassarri L, Orefici G, Creti R. A multiplex PCR assay for the direct identification of the capsular type (Ia to IX) of Streptococcus agalactiae. J Microbiol Methods. 2010; 80(2): 212-4[DOI][PubMed]
  • 15. Poyart C, Tazi A, Reglier-Poupet H, Billoet A, Tavares N, Raymond J, et al. Multiplex PCR assay for rapid and accurate capsular typing of group B streptococci. J Clin Microbiol. 2007; 45(6): 1985-8[DOI][PubMed]
  • 16. Brigtsen AK, Dedi L, Melby KK, Holberg-Petersen M, Radtke A, Lyng RV, et al. Comparison of PCR and serotyping of Group B Streptococcus in pregnant women: the Oslo GBS-study. J Microbiol Methods. 2015; 108: 31-5[DOI][PubMed]
  • 17. El Aila NA, Tency I, Claeys G, Saerens B, De Backer E, Temmerman M, et al. Genotyping of Streptococcus agalactiae (group B streptococci) isolated from vaginal and rectal swabs of women at 35-37 weeks of pregnancy. BMC Infect Dis. 2009; 9: 153[DOI][PubMed]
  • 18. Borchardt M, Foxman B, Chaffin DO, Rubens. C. E. , Tallman PA, Manning SD. Comparison of DNA Dot Blot Hybridization and Lancefield Capillary Precipitin Methods for Group B Streptococcal Capsular Typing. J Clin Microbiol. 2004; 42(1): 146-50[DOI]
  • 19. Dutra VG, Alves VM, Olendzki AN, Dias CA, de Bastos AF, Santos GO, et al. Streptococcus agalactiae in Brazil: serotype distribution, virulence determinants and antimicrobial susceptibility. BMC Infect Dis. 2014; 14: 323[DOI][PubMed]
  • 20. Kong F, Lambertsen LM, Slotved HC, Ko D, Wang H, Gilbert GL. Use of phenotypic and molecular serotype identification methods to characterize previously nonserotypeable group B streptococci. J Clin Microbiol. 2008; 46(8): 2745-50[DOI][PubMed]
  • 21. Tsolia M, Psoma M, Gavrili S, Petrochilou V, Michalas S, Legakis N, et al. Group B streptococcus colonization of Greek pregnant women and neonates: prevalence, risk factors and serotypes. Clin Microbiol Infect. 2003; 9(8): 832-8[PubMed]
  • 22. Brimil N, Barthell E, Heindrichs U, Kuhn M, Lutticken R, Spellerberg B. Epidemiology of Streptococcus agalactiae colonization in Germany. Int J Med Microbiol. 2006; 296(1): 39-44[DOI][PubMed]
  • 23. Ippolito DL, James WA, Tinnemore D, Huang RR, Dehart MJ, Williams J, et al. Group B streptococcus serotype prevalence in reproductive-age women at a tertiary care military medical center relative to global serotype distribution. BMC Infect Dis. 2010; 10: 336[DOI][PubMed]
  • 24. Lachenauer CS, Kasper DL, Shimada J, Ichiman Y, Ohtsuka H, Kaku M, et al. Serotypes VI and VIII predominate among group B streptococci isolated from pregnant Japanese women. J Infect Dis. 1999; 179(4): 1030-3[DOI][PubMed]
  • 25. Amin A, Abdulrazzaq YM, Uduman S. Group B streptococcal serotype distribution of isolates from colonized pregnant women at the time of delivery in United Arab Emirates. J Infect Dis. 2002; 45: 42-6[DOI]
  • 26. Jannati E, Roshani M, Arzanlou M, Habibzadeh S, Rahimi G, Shapuri R. Capsular serotype and antibiotic resistance of group B streptococci isolated from pregnant women in Ardabil, Iran. Iran J Microbiol. 2012; 4(3): 130-5[PubMed]
  • 27. Yasini M, Safari M, Khorshidi A, Moniri R, Mousavi GA, Samimi M. Frequency of group B capsular serotypes of Streptococcus using the multiplex PCR among the pregnant women in Kashan during 2011-2013. Feyz. 2013; 17(2): 173-80
  • 28. Nahaei MR, Ghandchilar N, Bilan N, Ghahramani P. Maternal Carriage and Neonatal Colonization of Streptococcus agalactiae in Tabriz, Northwest Iran. Iran J Med Sci. 2007; 32(3): 177-81
  • 29. Rahnama S, Forouhesh Tehrani H, Amirmozafari N, Azadmanesh K, Biglari S. Distribution of capsular serotypes in group B streptococci clinical isolates based on genotyping. Razi J Med Sci. 2010; 17(78 & 79): 25-33
  • 30. Afshar B, Broughton K, Creti R, Decheva A, Hufnagel M, Kriz P, et al. International external quality assurance for laboratory identification and typing of Streptococcus agalactiae (Group B streptococci). J Clin Microbiol. 2011; 49(4): 1475-82[DOI][PubMed]
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