Archives of Clinical Infectious Diseases

Published by: Kowsar

In Vitro Activity of Two Nanoparticles on Clinical Isolates of Candida parapsilosis, Showing Resistance Against Antifungal Agents in Children

Ensieh Lotfali 1 , Ahmad Reza Shahverdi 2 , Rasoul Mohammadi 3 , Fatemeh Noorbakhsh 4 , Ali Ghajari 1 , Saham Ansari 1 and Sassan Rezaie 5 , *
Authors Information
1 Department of Medical Parasitology and Mycology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
2 Department of Pharmaceutical Biotechnology and Biotechnology Research Centre, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
3 Department of Medical Parasitology and Mycology, School of Medicine, Infectious Disease and Tropical Medicine Research Center, Isfahan University of Medical Sciences, Isfahan, Iran
4 Department of Microbiology, Biological Science College, Varamin-Pishva Branch, Islamic Azad University, Varamin-Pishva, Iran
5 Division of Molecular Biology, Department of Medical Mycology and Parasitology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
Article information
  • Archives of Clinical Infectious Diseases: October 2017, 12 (4); e13853
  • Published Online: September 26, 2017
  • Article Type: Research Article
  • Received: January 23, 2017
  • Revised: May 22, 2017
  • Accepted: September 19, 2017
  • DOI: 10.5812/archcid.13853

To Cite: Lotfali E, Shahverdi A R, Mohammadi R, Noorbakhsh F, Ghajari A, et al. In Vitro Activity of Two Nanoparticles on Clinical Isolates of Candida parapsilosis, Showing Resistance Against Antifungal Agents in Children, Arch Clin Infect Dis. 2017 ;12(4):e13853. doi: 10.5812/archcid.13853.

Copyright: Copyright © 2017, Archives of Clinical Infectious Diseases. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License ( which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
1. Background
2. Methods
3. Results
4. Discussion
  • 1. Panacek A, Kolar M, Vecerova R, Prucek R, Soukupova J, Krystof V, et al. Antifungal activity of silver nanoparticles against Candida spp. Biomaterials. 2009;30(31):6333-40. doi: 10.1016/j.biomaterials.2009.07.065. [PubMed: 19698988].
  • 2. Peterson T. Treatment and prevention of fungal infection. focus on candidemia. NewYork: applied clinical education; 2007.
  • 3. Tay ST, Na SL, Chong J. Molecular differentiation and antifungal susceptibilities of Candida parapsilosis isolated from patients with bloodstream infections. J Med Microbiol. 2009;58(Pt 2):185-91. doi: 10.1099/jmm.0.004242-0. [PubMed: 19141735].
  • 4. Lotfi N, Shokohi T, Nouranibaladezaei SZ, Nasrolahi Omran A, Kondori N. High Recovery Rate of Non-albicans Candida Species Isolated From Burn Patients With Candidemia in Iran. Jundishapur J Microbiol. 2015;8(10). e22929. doi: 10.5812/jjm.22929. [PubMed: 26587207].
  • 5. Aghamirian MR, Ghiasian SA. Onychomycosis in Iran: epidemiology, causative agents and clinical features. Nihon Ishinkin Gakkai Zasshi. 2010;51(1):23-9. doi: 10.3314/jjmm.51.23. [PubMed: 20185868].
  • 6. Mahmoudi Rad M, Zafarghandi S, Abbasabadi B, Tavallaee M. The epidemiology of Candida species associated with vulvovaginal candidiasis in an Iranian patient population. Eur J Obstet Gynecol Reprod Biol. 2011;155(2):199-203. doi: 10.1016/j.ejogrb.2010.11.022. [PubMed: 21194828].
  • 7. White TC, Holleman S, Dy F, Mirels LF, Stevens DA. Resistance mechanisms in clinical isolates of Candida albicans. Antimicrob Agents Chemother. 2002;46(6):1704-13. doi: 10.1128/AAC.46.6.1704-1713.2002. [PubMed: 12019079].
  • 8. Dunkel N, Liu TT, Barker KS, Homayouni R, Morschhauser J, Rogers PD. A gain-of-function mutation in the transcription factor Upc2p causes upregulation of ergosterol biosynthesis genes and increased fluconazole resistance in a clinical Candida albicans isolate. Eukaryot Cell. 2008;7(7):1180-90. doi: 10.1128/EC.00103-08. [PubMed: 18487346].
  • 9. Kazempour ZB, Yazdi MH, Rafii F, Shahverdi AR. Sub-inhibitory concentration of biogenic selenium nanoparticles lacks post antifungal effect for Aspergillus niger and Candida albicans and stimulates the growth of Aspergillus niger. Iran J Microbiol. 2013;5(1):81-5. [PubMed: 23466957].
  • 10. Lipovsky A, Nitzan Y, Gedanken A, Lubart R. Antifungal activity of ZnO nanoparticles--the role of ROS mediated cell injury. Nanotechnology. 2011;22(10):105101. doi: 10.1088/0957-4484/22/10/105101. [PubMed: 21289395].
  • 11. Paulo CS, Vidal M, Ferreira LS. Antifungal nanoparticles and surfaces. Biomacromolecules. 2010;11(10):2810-7. doi: 10.1021/bm100893r. [PubMed: 20845938].
  • 12. Fallh F, Eslami G, Komali H, Houshmand A. Grapefruit alcoholic seed extract effect on Candida albicans resistant to fluconazole and clotrimazole. Int J Nutr Metab. 2010;2(3):56-9.
  • 13. Noorbakhsh F, Rezaie S, Shahverdi AR. Antifungal effects of silver nanoparticle alone and with combination of antifungal drug on dermatophyte pathogen Trichophyton rubrum. International Conference on Bioscience, Biochemistry and Bioinformatics. Singapore. 2011.
  • 14. Silver S. Bacterial silver resistance: molecular biology and uses and misuses of silver compounds. FEMS Microbiol Rev. 2003;27(2-3):341-53. doi: 10.1016/S0168-6445(03)00047-0. [PubMed: 12829274].
  • 15. Lotfali E, Kordbacheh P, Mirhendi H, Zaini F, Ghajari A, Mohammadi R, et al. Antifungal Susceptibility Analysis of Clinical Isolates of Candida parapsilosis in Iran. Iran J Public Health. 2016;45(3):322-8. [PubMed: 27141494].
  • 16. Wayne P. Clinical and laboratory standards institute. Implementation Guide of POCT for health care providers. 2007. p. 1-37.
  • 17. Fothergill AW, Sutton DA, McCarthy DI, Wiederhold NP. Impact of new antifungal breakpoints on antifungal resistance in Candida species. J Clin Microbiol. 2014;52(3):994-7. doi: 10.1128/JCM.03044-13. [PubMed: 24403302].
  • 18. Alimehr S, Shekari Ebrahim Abad H, Shahverdi AR, Hashemi J, Zomorodian K, Moazeni M, et al. Comparison of difference between fluconazole and silver nanoparticles in antimicrobial effect on fluconazole-resistant candida albicans strains. Arch Pediatr Infect Dis. 2015;3(2). doi: 10.5812/pedinfect.21481.
  • 19. Fesharaki PJ, Nazari P, Shakibaie M, Rezaie S, Banoee M, Abdollahi M, et al. Biosynthesis of selenium nanoparticles using Klebsiella pneumoniae and their recovery by a simple sterilization process. Braz J Microbiol. 2010;41(2):461-6. doi: 10.1590/S1517-838220100002000028. [PubMed: 24031517].
  • 20. Kim KJ, Sung WS, Moon SK, Choi JS, Kim JG, Lee DG. Antifungal effect of silver nanoparticles on dermatophytes. J Microbiol Biotechnol. 2008;18(8):1482-4. [PubMed: 18756112].
  • 21. Falahati M, Nozari S, Makhdoomi A, Ghasemi Z, Nami S, Assadi M. Comparison of antifungal effect of nanosilver particles alone and in combination with current drugs on candida species isolated from women with recurrent vulvovaginal candidiasis. Eur J Exp Biol. 2014;4(1):77-82.
  • 22. Nasrollahi A, Pourshamsian K, Mansourkiaee P. Antifungal activity of silver nanoparticles on some of fungi. Int J Nano Dimens. 2011;1(3):233-9.
  • 23. Wady AF, Machado AL, Foggi CC, Zamperini CA, Zucolotto V, Moffa EB, et al. Effect of a silver nanoparticles solution onstaphylococcus aureusand Candida spp. J Nanomater. 2014;2014:1-7. doi: 10.1155/2014/545279.
  • 24. Gajbhiye M, Kesharwani J, Ingle A, Gade A, Rai M. Fungus-mediated synthesis of silver nanoparticles and their activity against pathogenic fungi in combination with fluconazole. Nanomedicine. 2009;5(4):382-6. doi: 10.1016/j.nano.2009.06.005. [PubMed: 19616127].
  • 25. Gao C, Xu Y, Xu C. In vitro activity of nano-silver against ocular pathogenic fungi. Life Sci J. 2012;9(4):750-3.
  • 26. Ge L, Li Q, Wang M, Ouyang J, Li X, Xing MM. Nanosilver particles in medical applications: synthesis, performance, and toxicity. Int J Nanomedicine. 2014;9:2399-407. doi: 10.2147/IJN.S55015. [PubMed: 24876773].
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