Archives of Clinical Infectious Diseases

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Discrimination of Mixed Infections of Echinococcus Species Based on in Silico Sequence Analysis: A New Way of Reflecting Overlapped Strains in Indigenous Areas

Maryam Karimi 1 , Reza Ghasemikhah 2 , Hadi Mirahmadi 3 , Adel Spotin 4 , Soheila Rouhani 1 and Seyyed Javad Seyyed Tabaei 1 , *
Authors Information
1 Department of Parasitology and Mycology, Shahid Beheshti University of Medical Sciences, Tehran, Iran
2 Department of Parasitology and Mycology, Arak University of Medical Sciences, Arak, Iran
3 Department of Parasitology and Mycology, Zahedan University of Medical Sciences, Zahedan, Iran
4 Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
Article information
  • Archives of Clinical Infectious Diseases: October 2017, 12 (4); e14168
  • Published Online: September 30, 2017
  • Article Type: Research Article
  • Received: August 17, 2016
  • Revised: May 28, 2017
  • Accepted: September 19, 2017
  • DOI: 10.5812/archcid.14168

To Cite: Karimi M, Ghasemikhah R, Mirahmadi H, Spotin A, Rouhani S, et al. Discrimination of Mixed Infections of Echinococcus Species Based on in Silico Sequence Analysis: A New Way of Reflecting Overlapped Strains in Indigenous Areas, Arch Clin Infect Dis. 2017 ; 12(4):e14168. doi: 10.5812/archcid.14168.

Copyright © 2017, Archives of Clinical Infectious Diseases. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License ( which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited
1. Background
2. Methods
3. Results
4. Discussion
  • 1. Thompson RC, Jenkins DJ. Echinococcus as a model system: biology and epidemiology. Int J Parasitol. 2014;44(12):865-77. doi: 10.1016/j.ijpara.2014.07.005. [PubMed: 25123067].
  • 2. Thompson RC. Parasite zoonoses and wildlife: One Health, spillover and human activity. Int J Parasitol. 2013;43(12-13):1079-88. doi: 10.1016/j.ijpara.2013.06.007. [PubMed: 23892130].
  • 3. Thompson RC. The taxonomy, phylogeny and transmission of Echinococcus. Exp Parasitol. 2008;119(4):439-46. doi: 10.1016/j.exppara.2008.04.016. [PubMed: 18539274].
  • 4. Huttner M, Nakao M, Wassermann T, Siefert L, Boomker JD, Dinkel A, et al. Genetic characterization and phylogenetic position of Echinococcus felidis (Cestoda: Taeniidae) from the African lion. Int J Parasitol. 2008;38(7):861-8. doi: 10.1016/j.ijpara.2007.10.013. [PubMed: 18062973].
  • 5. Moks E, Jogisalu I, Valdmann H, Saarma U. First report of Echinococcus granulosus G8 in Eurasia and a reappraisal of the phylogenetic relationships of 'genotypes' G5-G10. Parasitology. 2008;135(5):647-54. doi: 10.1017/S0031182008004198. [PubMed: 18261256].
  • 6. Nakao M, McManus DP, Schantz PM, Craig PS, Ito A. A molecular phylogeny of the genus Echinococcus inferred from complete mitochondrial genomes. Parasitology. 2007;134(Pt 5):713-22. doi: 10.1017/S0031182006001934. [PubMed: 17156584].
  • 7. Nakao M, Lavikainen A, Yanagida T, Ito A. Phylogenetic systematics of the genus Echinococcus (Cestoda: Taeniidae). Int J Parasitol. 2013;43(12-13):1017-29. doi: 10.1016/j.ijpara.2013.06.002. [PubMed: 23872521].
  • 8. Casulli A, Manfredi MT, La Rosa G, Cerbo AR, Genchi C, Pozio E. Echinococcus ortleppi and E. granulosus G1, G2 and G3 genotypes in Italian bovines. Vet Parasitol. 2008;155(1-2):168-72. doi: 10.1016/j.vetpar.2008.04.004. [PubMed: 18514422].
  • 9. Spotin A, Gholami S, Nasab AN, Fallah E, Oskouei MM, Semnani V, et al. Designing and conducting in silico analysis for identifying of Echinococcus spp. with discrimination of novel haplotypes: an approach to better understanding of parasite taxonomic. Parasitol Res. 2015;114(4):1503-9. doi: 10.1007/s00436-015-4334-1. [PubMed: 25645007].
  • 10. Dakkak A. Echinococcosis/hydatidosis: a severe threat in Mediterranean countries. Vet Parasitol. 2010;174(1-2):2-11. doi: 10.1016/j.vetpar.2010.08.009. [PubMed: 20888694].
  • 11. Babiker AM, Ravagnan S, Fusaro A, Hassan MM, Bakheit SM, Mukhtar MM, et al. Concomitant infection with Leishmania donovani and L. majorin single ulcers of cutaneous leishmaniasis patients from Sudan. J Trop Med. 2014;2014:1-8. doi: 10.1155/2014/170859.
  • 12. Chang CT, Tsai CN, Tang CY, Chen CH, Lian JH, Hu CY, et al. Mixed sequence reader: a program for analyzing DNA sequences with heterozygous base calling. ScientificWorldJournal. 2012;2012:365104. doi: 10.1100/2012/365104. [PubMed: 22778697].
  • 13. Qvarnstrom Y, Visvesvara GS, Sriram R, da Silva AJ. Multiplex real-time PCR assay for simultaneous detection of Acanthamoeba spp., Balamuthia mandrillaris, and Naegleria fowleri. J Clin Microbiol. 2006;44(10):3589-95. doi: 10.1128/JCM.00875-06. [PubMed: 17021087].
  • 14. Hamzah Z, Petmitr S, Mungthin M, Leelayoova S, Chavalitshewinkoon-Petmitr P. Development of multiplex real-time polymerase chain reaction for detection of Entamoeba histolytica, Entamoeba dispar, and Entamoeba moshkovskii in clinical specimens. Am J Trop Med Hyg. 2010;83(4):909-13. doi: 10.4269/ajtmh.2010.10-0050. [PubMed: 20889890].
  • 15. Bowles J, McManus DP. Rapid discrimination of Echinococcus species and strains using a polymerase chain reaction-based RFLP method. Mol Biochem Parasitol. 1993;57(2):231-9. [PubMed: 8094539].
  • 16. Bowles J, Blair D, McManus DP. Genetic variants within the genus Echinococcus identified by mitochondrial DNA sequencing. Mol Biochem Parasitol. 1992;54(2):165-73. [PubMed: 1435857].
  • 17. Spotin A, Rouhani S, Parvizi P. The associations of Leishmania major and Leishmania tropica aspects by focusing their morphological and molecular features on clinical appearances in Khuzestan province, Iran. Biomed Res Int. 2014;2014:1-13. doi: 10.1155/2014/913510.
  • 18. Mahami Oskouei M, Ghabouli Mehrabani N, Miahipour A, Fallah E. Molecular characterization and sequence analysis of Echinococcus granulosus from sheep isolates in East Azerbaijan province, northwest of Iran. J Parasit Dis. 2016;40(3):785-90. doi: 10.1007/s12639-014-0579-3. [PubMed: 27605785].
  • 19. Parsa F, Fasihi Harandi M, Rostami S, Sharbatkhori M. Genotyping Echinococcus granulosus from dogs from Western Iran. Exp Parasitol. 2012;132(2):308-12. doi: 10.1016/j.exppara.2012.07.010. [PubMed: 22884512].
  • 20. Pezeshki A, Akhlaghi L, Sharbatkhori M, Razmjou E, Oormazdi H, Mohebali M, et al. Genotyping of Echinococcus granulosus from domestic animals and humans from Ardabil Province, northwest Iran. J Helminthol. 2013;87(4):387-91. doi: 10.1017/S0022149X1200051X. [PubMed: 23046636].
  • 21. Ahmadi N, Dalimi A. Characterization of Echinococcus granulosus isolates from human, sheep and camel in Iran. Infect Genet Evol. 2006;6(2):85-90. doi: 10.1016/j.meegid.2005.01.005. [PubMed: 16503509].
  • 22. Jamali R, Ghazanchaei A, Asgharzadeh M. Identification and characterization of Echinococcus granulosus by PCR RFLP technique in Tabriz district. J Parasitic Dis. 2004;28(2):69-72.
  • 23. Karimi A, Dianatpour R. Genotypic and phenotypic characterization of Echinococcus granulosus of Iran. Biotechnol. 2008;7(4):757-62. doi: 10.3923/biotech.2008.757.762.
  • 24. Rajabloo M, Hosseini SH, Jalousian F. Morphological and molecular characterisation of Echinococcus granulosus from goat isolates in Iran. Acta Trop. 2012;123(2):67-71. doi: 10.1016/j.actatropica.2012.03.006. [PubMed: 22484229].
  • 25. Smyth JD, McManus D, Barrett NJ, Bryceson A, Cowie AGA. In vitro culture of human hydatid material. Lancet. 1980;315(8161):202-3. doi: 10.1016/s0140-6736(80)90685-6.
  • 26. Clement M, Posada D, Crandall KA. TCS, a computer program to estimate gene genealogies. Mol Ecol. 2000;9(10):1657-9. [PubMed: 11050560].
  • 27. Huson DH, Bryant D. Application of phylogenetic networks in evolutionary studies. Mol Biol Evol. 2006;23(2):254-67. doi: 10.1093/molbev/msj030. [PubMed: 16221896].
  • 28. Youssefi MR, Tabaripour R, Omrani VF, Spotin A, Esfandiari B. Genotypic characterization of echinococcus granulosus in Iranian goats. Asian Pac J Trop Dis. 2013;3(5):362-6. doi: 10.1016/s2222-1808(13)60085-7.
  • 29. Umhang G, Richomme C, Boucher JM, Hormaz V, Boue F. Prevalence survey and first molecular characterization of Echinococcus granulosus in France. Parasitol Res. 2013;112(4):1809-12. doi: 10.1007/s00436-012-3245-7. [PubMed: 23271567].
  • 30. Shahnazi M, Hejazi H, Salehi M, Andalib AR. Molecular characterization of human and animal Echinococcus granulosus isolates in Isfahan, Iran. Acta Trop. 2011;117(1):47-50. doi: 10.1016/j.actatropica.2010.09.002. [PubMed: 20858453].
  • 31. Esposti MD, De Vries S, Crimi M, Ghelli A, Patarnello T, Meyer A. Mitochondrial cytochrome b: evolution and structure of the protein. Biochim Biophys Acta. 1993;1143(3):243-71. [PubMed: 8329437].
  • 32. Howell N, Gilbert K. Mutational analysis of the mouse mitochondrial cytochrome b gene. J Mol Biol. 1988;203(3):607-17. doi: 10.1016/0022-2836(88)90195-7.
  • 33. Mokhtari Amirmajdi M, Sankian M, Eftekharzadeh Mashhadi I, Varasteh A, Vahedi F, Sadrizadeh A, et al. Apoptosis of human lymphocytes after exposure to hydatid fluid, Mashhad university of medical sciences. Iran J Parasitol. 2011;6(2):9-16.
  • 34. Spotin A, Majdi MM, Sankian M, Varasteh A. The study of apoptotic bifunctional effects in relationship between host and parasite in cystic echinococcosis: a new approach to suppression and survival of hydatid cyst. Parasitol Res. 2012;110(5):1979-84. doi: 10.1007/s00436-011-2726-4. [PubMed: 22167369].
  • 35. Kamenetzky L, Canova SG, Guarnera EA, Rosenzvit MC. Echinococcus granulosus: DNA extraction from germinal layers allows strain determination in fertile and nonfertile hydatid cysts. Exp Parasitol. 2000;95(2):122-7. doi: 10.1006/expr.2000.4518. [PubMed: 10910713].
  • 36. Hajialilo E, Harandi MF, Sharbatkhori M, Mirhendi H, Rostami S. Genetic characterization of Echinococcus granulosus in camels, cattle and sheep from the south-east of Iran indicates the presence of the G3 genotype. J Helminthol. 2012;86(3):263-70. doi: 10.1017/S0022149X11000320. [PubMed: 21749740].
  • 37. Shariatzadeh SA, Spotin A, Gholami S, Fallah E, Hazratian T, Mahami Oskouei M, et al. The first morphometric and phylogenetic perspective on molecular epidemiology of Echinococcus granulosus sensu lato in stray dogs in a hyperendemic Middle East focus, northwestern Iran. Parasit Vectors. 2015;8:409. doi: 10.1186/s13071-015-1025-9. [PubMed: 26242729].

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