Archives of Clinical Infectious Diseases

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Molecular Investigation of Methicillin-Resistant Staphylococcus aureus Strains Recovered from the Intensive Care Unit (ICU) Based on Toxin, Adhesion Genes and agr Locus Type Analysis

Sara Nasirian 1 , 2 , Sara Saadatmand 2 , Hossein Goudarzi 1 , Mehdi Goudarzi 1 , * and Hadi Azimi 3
Authors Information
1 Department of Microbiology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
2 Department of Biology, Faculty of Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran
3 Department of English Language Teaching, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Article information
  • Archives of Clinical Infectious Diseases: April 2018, 13 (2); e14495
  • Published Online: April 24, 2018
  • Article Type: Research Article
  • Received: January 3, 2017
  • Revised: August 13, 2017
  • Accepted: September 19, 2017
  • DOI: 10.5812/archcid.14495

To Cite: Nasirian S, Saadatmand S, Goudarzi H, Goudarzi M, Azimi H. Molecular Investigation of Methicillin-Resistant Staphylococcus aureus Strains Recovered from the Intensive Care Unit (ICU) Based on Toxin, Adhesion Genes and agr Locus Type Analysis, Arch Clin Infect Dis. 2018 ; 13(2):e14495. doi: 10.5812/archcid.14495.

Abstract
Copyright © 2018, Archives of Clinical Infectious Diseases. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited
1. Background
2. Objectives
3. Methods
4. Results
5. Discussion
Acknowledgements
References
  • 1. Gordon RJ, Lowy FD. Pathogenesis of methicillin-resistant Staphylococcus aureus infection. Clin Infect Dis. 2008;46 Suppl 5:S350-9. doi: 10.1086/533591. [PubMed: 18462090]. [PubMed Central: PMC2474459].
  • 2. Goudarzi M, Goudarzi H, Sa Figueiredo AM, Udo EE, Fazeli M, Asadzadeh M, et al. Molecular Characterization of Methicillin Resistant Staphylococcus aureus Strains Isolated from Intensive Care Units in Iran: ST22-SCCmec IV/t790 Emerges as the Major Clone. PLoS One. 2016;11(5). e0155529. doi: 10.1371/journal.pone.0155529. [PubMed: 27171373]. [PubMed Central: PMC4865093].
  • 3. Jevons MP. "Celbenin" - resistant Staphylococci. Br Med J. 1961;1(5219):124-5. doi: 10.1136/bmj.1.5219.124-a.
  • 4. Dulon M, Haamann F, Peters C, Schablon A, Nienhaus A. MRSA prevalence in European healthcare settings: a review. BMC Infect Dis. 2011;11:138. doi: 10.1186/1471-2334-11-138. [PubMed: 21599908]. [PubMed Central: PMC3128047].
  • 5. Goudarzi M, Seyedjavadi SS, Nasiri MJ, Goudarzi H, Sajadi Nia R, Dabiri H. Molecular characteristics of methicillin-resistant Staphylococcus aureus (MRSA) strains isolated from patients with bacteremia based on MLST, SCCmec, spa, and agr locus types analysis. Microb Pathog. 2017;104:328-35. doi: 10.1016/j.micpath.2017.01.055. [PubMed: 28159661].
  • 6. Eftekhar F, Rezaee R, Azad M, Azimi H, Goudarzi H, Goudarzi M. Distribution of adhesion and toxin genes in staphylococcus aureus strains recovered from hospitalized patients admitted to the ICU. Arch Ped Infect Dis. 2016;5(1). doi: 10.5812/pedinfect.39349.
  • 7. Gilot P, Lina G, Cochard T, Poutrel B. Analysis of the genetic variability of genes encoding the RNA III-activating components Agr and TRAP in a population of Staphylococcus aureus strains isolated from cows with mastitis. J Clin Microbiol. 2002;40(11):4060-7. doi: 10.1128/JCM.40.11.4060-4067.2002. [PubMed: 12409375]. [PubMed Central: PMC139642].
  • 8. Tsompanidou E, Sibbald MJ, Chlebowicz MA, Dreisbach A, Back JW, van Dijl JM, et al. Requirement of the agr locus for colony spreading of Staphylococcus aureus. J Bacteriol. 2011;193(5):1267-72. doi: 10.1128/JB.01276-10. [PubMed: 21169484]. [PubMed Central: PMC3067592].
  • 9. Goudarzi M, Seyedjavadi SS, Azad M, Goudarzi H, Azimi H. Distribution of spa types, integrons and associated gene cassettes in staphylococcus aureus strains isolated from intensive care units of hospitals in Tehran, Iran. Arch Clin Infect Dis. 2016;11(4). doi: 10.5812/archcid.38813.
  • 10. Clinical and Laboratory Standards Institute . Performance standards for antimicrobial susceptibility testing; Twenty-Second Informational Supplement. CLSI; 2016.
  • 11. Jarraud S, Mougel C, Thioulouse J, Lina G, Meugnier H, Forey F, et al. Relationships between Staphylococcus aureus genetic background, virulence factors, agr groups (alleles), and human disease. Infect Immun. 2002;70(2):631-41. doi: 10.1128/IAI.70.2.631-641.2002. [PubMed: 11796592]. [PubMed Central: PMC127674].
  • 12. Hoseini Alfatemi SM, Motamedifar M, Hadi N, Sedigh Ebrahim Saraie H. Analysis of Virulence Genes Among Methicillin Resistant Staphylococcus aureus (MRSA) Strains. Jundishapur J Microbiol. 2014;7(6). e10741. doi: 10.5812/jjm.10741. [PubMed: 25371805]. [PubMed Central: PMC4217665].
  • 13. Ghasemian A, Najar Peerayeh S, Bakhshi B, Mirzaee M. Several virulence factors of multidrug-resistant staphylococcus aureus isolates from hospitalized patients in Tehran. Int J Enteric Pathog. 2015;3(2). doi: 10.17795/ijep25196.
  • 14. Kumar JD, Negi YK, Gaur A, Khanna D. Detection of virulence genes in Staphylococcus aureus isolated from paper currency. Int J Infect Dis. 2009;13(6):e450-5. doi: 10.1016/j.ijid.2009.02.020. [PubMed: 19477670].
  • 15. Peacock SJ, Moore CE, Justice A, Kantzanou M, Story L, Mackie K, et al. Virulent combinations of adhesin and toxin genes in natural populations of Staphylococcus aureus. Infect Immun. 2002;70(9):4987-96. doi: 10.1128/IAI.70.9.4987-4996.2002. [PubMed: 12183545]. [PubMed Central: PMC128268].
  • 16. Rashidi Nezhad R, Meybodi SM, Rezaee R, Goudarzi M, Fazeli M. Molecular characterization and resistance profile of methicillin resistant staphylococcus aureus strains isolated from hospitalized patients in intensive care unit, Tehran-Iran. Jundishapur J Microbiol. 2017;10(3). doi: 10.5812/jjm.41666.
  • 17. Goudarzi M, Fazeli M, Goudarzi H, Azad M, Seyedjavadi SS. Spa Typing of Staphylococcus aureus Strains Isolated From Clinical Specimens of Patients With Nosocomial Infections in Tehran, Iran. Jundishapur J Microbiol. 2016;9(7). e35685. doi: 10.5812/jjm.35685. [PubMed: 27679706]. [PubMed Central: PMC5035396].
  • 18. Guney AK. A study on class i integrons and antimicrobial resistance among clinical staphylococci isolates from a Turkish hospital. Clin Microbiol. 2014;3(6):173. doi: 10.4172/2327-5073.1000173.
  • 19. Campanile F, Bongiorno D, Borbone S, Stefani S. Hospital-associated methicillin-resistant Staphylococcus aureus (HA-MRSA) in Italy. Ann Clin Microbiol Antimicrob. 2009;8:22. doi: 10.1186/1476-0711-8-22. [PubMed: 19552801]. [PubMed Central: PMC2708121].
  • 20. Dormanesh B, Siroosbakhat S, Khodaverdi Darian E, Afsharkhas L. Methicillin-Resistant Staphylococcus aureus Isolated From Various Types of Hospital Infections in Pediatrics: Panton-Valentine Leukocidin, Staphylococcal Chromosomal Cassette mec SCCmec Phenotypes and Antibiotic Resistance Properties. Jundishapur J Microbiol. 2015;8(11). e11341. doi: 10.5812/jjm.11341. [PubMed: 26862375]. [PubMed Central: PMC4741056].
  • 21. Havaei SA, Ghanbari F, Rastegari AA, Azimian A, Khademi F, Hosseini N, et al. Molecular Typing of Hospital-Acquired Staphylococcus aureus Isolated from Isfahan, Iran. Int Sch Res Notices. 2014;2014:185272. doi: 10.1155/2014/185272. [PubMed: 27350987]. [PubMed Central: PMC4897504].
  • 22. Wang WY, Chiueh TS, Sun JR, Tsao SM, Lu JJ. Molecular typing and phenotype characterization of methicillin-resistant Staphylococcus aureus isolates from blood in Taiwan. PLoS One. 2012;7(1). e30394. doi: 10.1371/journal.pone.0030394. [PubMed: 22291948]. [PubMed Central: PMC3264593].
  • 23. Ko KS, Lee JY, Suh JY, Oh WS, Peck KR, Lee NY, et al. Distribution of major genotypes among methicillin-resistant Staphylococcus aureus clones in Asian countries. J Clin Microbiol. 2005;43(1):421-6. doi: 10.1128/JCM.43.1.421-426.2005. [PubMed: 15635004]. [PubMed Central: PMC540159].
  • 24. Goudarzi M, Bahramian M, Satarzadeh Tabrizi M, Udo EE, Figueiredo AM, Fazeli M, et al. Genetic diversity of methicillin resistant Staphylococcus aureus strains isolated from burn patients in Iran: ST239-SCCmec III/t037 emerges as the major clone. Microb Pathog. 2017;105:1-7. doi: 10.1016/j.micpath.2017.02.004. [PubMed: 28179118].
  • 25. Gadepalli R, Dhawan B, Mohanty S, Kapil A, Das BK, Chaudhry R, et al. Mupirocin resistance in Staphylococcus aureus in an Indian hospital. Diagn Microbiol Infect Dis. 2007;58(1):125-7. doi: 10.1016/j.diagmicrobio.2006.10.012. [PubMed: 17240103].
  • 26. Aqel AA, Ibrahim A, Shehabi A. Rare occurrence of mupirocin resistance among clinical Staphylococcus isolates in Jordan. Acta Microbiol Immunol Hung. 2012;59(2):239-47. doi: 10.1556/AMicr.59.2012.2.8. [PubMed: 22750783].
  • 27. Petinaki E, Spiliopoulou I, Kontos F, Maniati M, Bersos Z, Stakias N, et al. Clonal dissemination of mupirocin-resistant staphylococci in Greek hospitals. J Antimicrob Chemother. 2004;53(1):105-8. doi: 10.1093/jac/dkh028. [PubMed: 14657085].
  • 28. Abbasi-Montazeri E, Khosravi AD, Feizabadi MM, Goodarzi H, Khoramrooz SS, Mirzaii M, et al. The prevalence of methicillin resistant Staphylococcus aureus (MRSA) isolates with high-level mupirocin resistance from patients and personnel in a burn center. Burns. 2013;39(4):650-4. doi: 10.1016/j.burns.2013.02.005. [PubMed: 23499497].
  • 29. Debdas D, Joshi S. Incidence of clindamycin resistance in clinical isolates of Staphylococcus aureus. J Infect Dev Ctries. 2011;5(4):316-7. doi: 10.3855/jidc.1598. [PubMed: 21537077].
  • 30. Schreckenberger PC, Ilendo E, Ristow KL. Incidence of constitutive and inducible clindamycin resistance in Staphylococcus aureus and coagulase-negative staphylococci in a community and a tertiary care hospital. J Clin Microbiol. 2004;42(6):2777-9. doi: 10.1128/JCM.42.6.2777-2779.2004. [PubMed: 15184468]. [PubMed Central: PMC427875].
  • 31. Ghanbari F, Ghajavand H, Havaei R, Jami MS, Khademi F, Heydari L, et al. Distribution of erm genes among Staphylococcus aureus isolates with inducible resistance to clindamycin in Isfahan, Iran. Adv Biomed Res. 2016;5:62. doi: 10.4103/2277-9175.179184. [PubMed: 27135031]. [PubMed Central: PMC4832884].
  • 32. Jimenez JN, Ocampo AM, Vanegas JM, Rodriguez EA, Garces CG, Patino LA, et al. Characterisation of virulence genes in methicillin susceptible and resistant Staphylococcus aureus isolates from a paediatric population in a university hospital of Medellin, Colombia. Mem Inst Oswaldo Cruz. 2011;106(8):980-5. doi: 10.1590/S0074-02762011000800013. [PubMed: 22241120].
  • 33. Lim KT, Hanifah YA, Mohd Yusof MY, Thong KL. Investigation of toxin genes among methicillin-resistant Staphylococcus aureus strains isolated from a tertiary hospital in Malaysia. Trop Biomed. 2012;29(2):212-9. [PubMed: 22735842].
  • 34. Nowrouzian FL, Dauwalder O, Meugnier H, Bes M, Etienne J, Vandenesch F, et al. Adhesin and superantigen genes and the capacity of Staphylococcus aureus to colonize the infantile gut. J Infect Dis. 2011;204(5):714-21. doi: 10.1093/infdis/jir388. [PubMed: 21844297].
  • 35. Sila J, Sauer P, Kolar M. Comparison of the prevalence of genes coding for enterotoxins, exfoliatins, panton-valentine leukocidin and tsst-1 between methicillin-resistant and methicillin-susceptible isolates of Staphylococcus aureus at the university hospital in olomouc. Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2009;153(3):215-8. doi: 10.5507/bp.2009.036. [PubMed: 19851435].
  • 36. Demir C, Aslantaş, Ö , Duran, N , Ocak, S , Özer, B . Investigation of toxin genes in Staphylococcus aureus strains isolated in Mustafa Kemal University hospital. Turk J Med Sci. 2011;41(2):434-52. doi: 10.3906/sag-1003-657.
  • 37. Indrawattana N, Sungkhachat O, Sookrung N, Chongsa-nguan M, Tungtrongchitr A, Voravuthikunchai SP, et al. Staphylococcus aureus clinical isolates: antibiotic susceptibility, molecular characteristics, and ability to form biofilm. Biomed Res Int. 2013;2013:314654. doi: 10.1155/2013/314654. [PubMed: 24069597]. [PubMed Central: PMC3773402].
  • 38. Ben Ayed S, Boutiba-Ben Boubaker I, Ennigrou S, Ben Redjeb S. Accessory gene regulator (agr) typing of Staphylococcus aureus isolated from human infections. Arch Inst Pasteur Tunis. 2008;85(1-4):3-8. [PubMed: 19469411].
  • 39. Ghasemian A, Peerayeh SN, Bakhshi B, Mirzaee M. Detection of accessory gene regulator groups genes and cassette chromosome mec types among Staphylococcus aureus isolated from intensive care unit patients. Asian Pac J Trop Dis. 2015;5(2):153-7. doi: 10.1016/s2222-1808(14)60643-5.
  • 40. Shopsin B, Mathema B, Alcabes P, Said-Salim B, Lina G, Matsuka A, et al. Prevalence of agr specificity groups among Staphylococcus aureus strains colonizing children and their guardians. J Clin Microbiol. 2003;41(1):456-9. doi: 10.1128/JCM.41.1.456-459.2003. [PubMed: 12517893]. [PubMed Central: PMC149583].

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