Archives of Clinical Infectious Diseases

Published by: Kowsar

Isolation of Methicillin-Resistant Staphylococcus aureus Strains Producing Enterotoxins A, K and Q From Chicken Meat in Isfahan, Iran, 2014

Fateh Rahimi 1 , * and Sharmin Karimi 1
Authors Information
1 Department of Microbiology, Faculty of Science, University of Isfahan, Isfahan, IR Iran
Article information
  • Archives of Clinical Infectious Diseases: October 01, 2016, 11 (4); e35601
  • Published Online: August 27, 2016
  • Article Type: Research Article
  • Received: December 18, 2015
  • Revised: August 8, 2016
  • Accepted: August 20, 2016
  • DOI: 10.5812/archcid.35601

To Cite: Rahimi F, Karimi S. Isolation of Methicillin-Resistant Staphylococcus aureus Strains Producing Enterotoxins A, K and Q From Chicken Meat in Isfahan, Iran, 2014, Arch Clin Infect Dis. 2016 ; 11(4):e35601. doi: 10.5812/archcid.35601.

Copyright © 2016, Infectious Diseases and Tropical Medicine Research Center. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License ( which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
1. Background
2. Objectives
3. Methods
4. Results
5. Discussion
  • 1. Argudin MA, Mendoza MC, Rodicio MR. Food poisoning and Staphylococcus aureus enterotoxins. Toxins (Basel). 2010; 2(7): 1751-73[DOI][PubMed]
  • 2. Ortega E, Abriouel H, Lucas R, Galvez A. Multiple roles of Staphylococcus aureus enterotoxins: pathogenicity, superantigenic activity, and correlation to antibiotic resistance. Toxins (Basel). 2010; 2(8): 2117-31[DOI][PubMed]
  • 3. Kerouanton A, Hennekinne JA, Letertre C, Petit L, Chesneau O, Brisabois A, et al. Characterization of Staphylococcus aureus strains associated with food poisoning outbreaks in France. Int J Food Microbiol. 2007; 115(3): 369-75[DOI][PubMed]
  • 4. Schelin J, Wallin-Carlquist N, Cohn MT, Lindqvist R, Barker GC, Radstrom P. The formation of Staphylococcus aureus enterotoxin in food environments and advances in risk assessment. Virulence. 2011; 2(6): 580-92[DOI][PubMed]
  • 5. Malachowa N, DeLeo FR. Mobile genetic elements of Staphylococcus aureus. Cell Mol Life Sci. 2010; 67(18): 3057-71[DOI][PubMed]
  • 6. Rahimi F, Bouzari M, Katouli M, Pourshafie M. Prophage typing of methicillin resistant Staphylococcus aureus isolated from a tertiary care hospital in Tehran, Iran. Jundishapur J Microbiol. 2013; 6(1): 80-5
  • 7. Rahimi F, Bouzari M, Katouli M, Pourshafie MR. Prophage and antibiotic resistance profiles of methicillin-resistant Staphylococcus aureus strains in Iran. Arch Virol. 2012; 157(9): 1807-11[DOI][PubMed]
  • 8. Pantucek R, Doskar J, Ruzickova V, Kasparek P, Oracova E, Kvardova V, et al. Identification of bacteriophage types and their carriage in Staphylococcus aureus. Arch Virol. 2004; 149(9): 1689-703[PubMed]
  • 9. Rahimi F, Katouli M, Pourshafie MR. Characteristics of hospital- and community-acquired meticillin-resistant Staphylococcus aureus in Tehran, Iran. J Med Microbiol. 2014; 63: 796-804[DOI][PubMed]
  • 10. Rahimi F, Karimi S. Characteristics of methicillin resistant staphylococcus aureus strains isolated from poultry in iran. Arch Clin Infect Dis. 2015; 10(4)[DOI]
  • 11. Talebi M, Sadeghi J, Rahimi F, Pourshafie MR. Isolation and Biochemical Fingerprinting of Vancomycin-Resistant Enterococcus faecium From Meat, Chicken and Cheese. Jundishapur J Microbiol. 2015; 8(4): 15815[DOI][PubMed]
  • 12. Zouharova M, Rysanek D. Multiplex PCR and RPLA Identification of Staphylococcus aureus enterotoxigenic strains from bulk tank milk. Zoonoses Public Health. 2008; 55(6): 313-9[DOI][PubMed]
  • 13. McClure JA, Conly JM, Lau V, Elsayed S, Louie T, Hutchins W, et al. Novel multiplex PCR assay for detection of the staphylococcal virulence marker Panton-Valentine leukocidin genes and simultaneous discrimination of methicillin-susceptible from -resistant staphylococci. J Clin Microbiol. 2006; 44(3): 1141-4[DOI][PubMed]
  • 14. Zhang K, McClure JA, Elsayed S, Louie T, Conly JM. Novel multiplex PCR assay for characterization and concomitant subtyping of staphylococcal cassette chromosome mec types I to V in methicillin-resistant Staphylococcus aureus. J Clin Microbiol. 2005; 43(10): 5026-33[DOI][PubMed]
  • 15. Sergeev N, Volokhov D, Chizhikov V, Rasooly A. Simultaneous analysis of multiple staphylococcal enterotoxin genes by an oligonucleotide microarray assay. J Clin Microbiol. 2004; 42(5): 2134-43[PubMed]
  • 16. Bhargava K, Wang X, Donabedian S, Zervos M, de Rocha L, Zhang Y. Methicillin-resistant Staphylococcus aureus in retail meat, Detroit, Michigan, USA. Emerg Infect Dis. 2011; 17(6): 1135-7[DOI][PubMed]
  • 17. de Boer E, Zwartkruis-Nahuis JT, Wit B, Huijsdens XW, de Neeling AJ, Bosch T, et al. Prevalence of methicillin-resistant Staphylococcus aureus in meat. Int J Food Microbiol. 2009; 134(1-2): 52-6[DOI][PubMed]
  • 18. Hanson BM, Dressler AE, Harper AL, Scheibel RP, Wardyn SE, Roberts LK, et al. Prevalence of Staphylococcus aureus and methicillin-resistant Staphylococcus aureus (MRSA) on retail meat in Iowa. J Infect Public Health. 2011; 4(4): 169-74[DOI][PubMed]
  • 19. Jackson CR, Davis JA, Barrett JB. Prevalence and characterization of methicillin-resistant Staphylococcus aureus isolates from retail meat and humans in Georgia. J Clin Microbiol. 2013; 51(4): 1199-207[DOI][PubMed]
  • 20. Lozano C, Lopez M, Gomez-Sanz E, Ruiz-Larrea F, Torres C, Zarazaga M. Detection of methicillin-resistant Staphylococcus aureus ST398 in food samples of animal origin in Spain. J Antimicrob Chemother. 2009; 64(6): 1325-6[DOI][PubMed]
  • 21. Pu S, Han F, Ge B. Isolation and characterization of methicillin-resistant Staphylococcus aureus strains from Louisiana retail meats. Appl Environ Microbiol. 2009; 75(1): 265-7[DOI][PubMed]
  • 22. van Loo IH, Diederen BM, Savelkoul PH, Woudenberg JH, Roosendaal R, van Belkum A, et al. Methicillin-resistant Staphylococcus aureus in meat products, the Netherlands. Emerg Infect Dis. 2007; 13(11): 1753-5[DOI][PubMed]
  • 23. Weese JS, Reid-Smith R, Rousseau J, Avery B. Methicillin-resistant Staphylococcus aureus (MRSA) contamination of retail pork. Can Vet J. 2010; 51(7): 749-52[PubMed]
  • 24. Lozano C, Gharsa H, Ben Slama K, Zarazaga M, Torres C. Staphylococcus aureus in animals and food: Methicillin resistance, prevalence and population structure. A review in the african continent. Microorganisms. 2016; 4(1): 12[DOI]
  • 25. Fatholahzadeh B, Emaneini M, Gilbert G, Udo E, Aligholi M, Modarressi MH, et al. Staphylococcal cassette chromosome mec (SCCmec) analysis and antimicrobial susceptibility patterns of methicillin-resistant Staphylococcus aureus (MRSA) isolates in Tehran, Iran. Microb Drug Resist. 2008; 14(3): 217-20[DOI][PubMed]
  • 26. Japoni A, Jamalidoust M, Farshad S, Ziyaeyan M, Alborzi A, Japoni S, et al. Characterization of SCCmec types and antibacterial susceptibility patterns of methicillin-resistant Staphylococcus aureus in Southern Iran. Jpn J Infect Dis. 2011; 64(1): 28-33[PubMed]
  • 27. Rahimi F, Bouzari M. Biochemical Fingerprinting of Methicillin-Resistant Staphylococcus aureus Isolated From Sewage and Hospital in Iran. Jundishapur J Microbiol. 2015; 8(7): 19760[DOI][PubMed]
  • 28. Rahimi F. Characterization of Resistance to Aminoglycosides in Methicillin-Resistant Staphylococcus aureus Strains Isolated From a Tertiary Care Hospital in Tehran, Iran. Jundishapur J Microbiol. 2016; 9(1): 29237[DOI][PubMed]
  • 29. Rahimi F, Katouli M, Karimi S. Biofilm production among methicillin resistant Staphylococcus aureus strains isolated from catheterized patients with urinary tract infection. Microb Pathog. 2016; 98: 69-76[DOI][PubMed]
  • 30. Rahimi F, Shokoohizadeh L. Characterization of methicillin resistant Staphylococcus aureus strains among inpatients and outpatients in a referral hospital in Tehran, Iran. Microb Pathog. 2016; 97: 89-93[DOI][PubMed]
  • 31. Workman M, Nigro O, Steward G. Identification of prophages in coastal water isolates of staphylococcus aureus. J Young Invest. 2006; 15: 1-8
  • 32. Alibayov B, Zdenkova K, Sykorova H, Demnerova K. Molecular analysis of Staphylococcus aureus pathogenicity islands (SaPI) and their superantigens combination of food samples. J Microbiol Methods. 2014; 107: 197-204[DOI][PubMed]
  • 33. Aydin A, Sudagidan M, Muratoglu K. Prevalence of staphylococcal enterotoxins, toxin genes and genetic-relatedness of foodborne Staphylococcus aureus strains isolated in the Marmara Region of Turkey. Int J Food Microbiol. 2011; 148(2): 99-106[DOI][PubMed]
  • 34. Carfora V, Caprioli A, Marri N, Sagrafoli D, Boselli C, Giacinti G, et al. Enterotoxin genes, enterotoxin production, and methicillin resistance in staphylococcus aureus isolated from milk and dairy products in central Italy. Int Dairy J. 2015; 42: 12-5
  • 35. Chao G, Bao G, Cao Y, Yan W, Wang Y, Zhang X, et al. Prevalence and diversity of enterotoxin genes with genetic background of Staphylococcus aureus isolates from different origins in China. Int J Food Microbiol. 2015; 211: 142-7[DOI][PubMed]
  • 36. Jarraud S, Peyrat MA, Lim A, Tristan A, Bes M, Mougel C, et al. egc, a highly prevalent operon of enterotoxin gene, forms a putative nursery of superantigens in Staphylococcus aureus. J Immunol. 2001; 166(1): 669-77[PubMed]
  • 37. Omoe K, Ishikawa M, Shimoda Y, Hu DL, Ueda S, Shinagawa K. Detection of seg, seh, and sei genes in Staphylococcus aureus isolates and determination of the enterotoxin productivities of S. aureus isolates Harboring seg, seh, or sei genes. J Clin Microbiol. 2002; 40(3): 857-62[PubMed]
  • 38. Saadati M, Barati B, Doroudian M, Shirzad H, Hashemi M, Hosseini SM, et al. Detection of Sea, Seb, Sec, Seq genes in staphylococcus aureus isolated from nasal carriers in Tehran province, Iran; by multiplex PCR. J Paramed Sci. 2011; 2(2)
  • 39. Smyth DS, Hartigan PJ, Meaney WJ, Fitzgerald JR, Deobald CF, Bohach GA, et al. Superantigen genes encoded by the egc cluster and SaPIbov are predominant among Staphylococcus aureus isolates from cows, goats, sheep, rabbits and poultry. J Med Microbiol. 2005; 54: 401-11[DOI][PubMed]
  • 40. Spanu V, Spanu C, Virdis S, Cossu F, Scarano C, De Santis EP. Virulence factors and genetic variability of Staphylococcus aureus strains isolated from raw sheep's milk cheese. Int J Food Microbiol. 2012; 153(1-2): 53-7[DOI][PubMed]
  • 41. Xie Y, He Y, Gehring A, Hu Y, Li Q, Tu SI, et al. Genotypes and toxin gene profiles of Staphylococcus aureus clinical isolates from China. PLoS One. 2011; 6(12): 28276[DOI][PubMed]
  • 42. Rahimi F, Karimi S. Characteristics of virulence factors in methicillin-resistant staphylococcus aureus strains isolated from a referral hospital in Tehran, Iran. Arch Clin Infect Dis. 2016; 11(1)
Creative Commons License Except where otherwise noted, this work is licensed under Creative Commons Attribution Non Commercial 4.0 International License .
Readers' Comments