Cite Score:
0.73
CITE SCORE SCOPUS

Detection of Mycobacterium Tuberculosis Complex by gyrB PCR in Patients with Clinical Suspicious of Tuberculosis in Mazandaran, Iran

AUTHORS

Maryam Pourhajibagher 1 , Mohtaram Nasrollahi 2 , * , Mohammad Ahanjan 3

1 MSc Student of Microbiology, Faculty of Medicine, Mazandaran University of Medical Sciences, IR Iran

2 Professor of Microbiology Faculty of Medicine, Mazandaran University of Medical Sciences, IR Iran, mphb65@yahoo.com

3 Assistant Professor of Microbiology, Faculty of Medicine. Mazandaran University of Medical Sciences, Iran

How to Cite: Pourhajibagher M, Nasrollahi M, Ahanjan M. Detection of Mycobacterium Tuberculosis Complex by gyrB PCR in Patients with Clinical Suspicious of Tuberculosis in Mazandaran, Iran, Arch Clin Infect Dis. Online ahead of Print ; 6(3):104-7.

ARTICLE INFORMATION

Archives of Clinical Infectious Diseases: 6 (3); 104-7
Article Type: Research Article

Crossmark

CHEKING

READ FULL TEXT
Abstract

Introduction: Mycobacterium tuberculosis complex (MTBC) are causative agents of human and animal tuberculosis.Differentiation of MTBC members is required for appropriate treatment of individual patients and for epidemiologicalpurposes and reduction in drug resistances.

Material and Methods: 1345 patients were collected with clinical suspicions of tuberculosis who referred to the Health Care Center of Mazandaran province from July 2010 to June 2011. The specimens were stained by the Ziehl-Neelsen staining technique and were cultured on Lowenstein-Jensen medium to detect the mycobacteria. For recognition of Mycobacterium tuberculosis complex MTUB-f and MTUB-r primer (gyrB-PCR1) were used. For differentiation of Mycobacterium tuberculosis complex members MTUB-756-Gf and MTUB- 1450Cr (gyrB-PCR2) and RFLP PCR using RsaI restriction enzymewere used.

Results: Of 1345 specimens, only 65(4.83%) isolates were positive culture of which59 (90.76%) were MTBC and 6 (9.24%) identified as Mycobacteria other than tuberculosis. All of 59 isolates were M. tuberculosis.

Conclusion: The gyrB-RFLP PCR and using the RsaI restriction enzyme is a rapid and easy technique to perform for differentiation of the member of M. tuberculosis complex.

Keywords

gyrB, RFLP PCR, RsaI, Mycobacterium tuberculosis complex

© 0, Archives of Clinical Infectious Diseases. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.

Full Text

Full text is available in PDF

COMMENTS

LEAVE A COMMENT HERE: