Evaluation of a PCR Based Approach to Study the Relatedness Among Shigella sonnei Strains

AUTHORS

Reza Ranjbar 1 , * , Nourkhoda Sadeghifard 2 , Mohammad Mahdi Soltan Dallal 3 , Shohreh Farshad 4

1 Molecular Biology Research Center, Baqiyatallah University of Medical Sciences, Tehran, IR Iran

2 Department of Microbiology, Faculty of Medicine, Ilam University of Medical Sciences, Ilam, IR Iran

3 Department of Pathobiology, School of Public Health and Institute of Public Health Research, Tehran University of Medical Science, Tehran, IR Iran

4 Professor Alborzi Clinical Microbiology Research Center, Shiraz University of Medical Sciences, Shiraz, IR Iran

How to Cite: Ranjbar R, Sadeghifard N, Soltan Dallal M M, Farshad S. Evaluation of a PCR Based Approach to Study the Relatedness Among Shigella sonnei Strains, Arch Clin Infect Dis. Online ahead of Print ; 4(3):163-6.

ARTICLE INFORMATION

Archives of Clinical Infectious Diseases: 4 (3); 163-6
Article Type: Research Article

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Abstract

Background: Infections caused by Shigella are a major cause of diarrheal disease in the developing and developed countries. The present study was conducted to apply and evaluate arbitrarily primed PCR (AP-PCR) for investigation of genetic relatedness among the strains of Shigella sonnei isolated from cases of acute diarrhea in Tehran.

Patients and methods: Totally, 60 S. sonnei strains isolated from children hospitalized due to enteritis at five hospitals in Tehran during 2003 and two sporadic isolates recovered in 1984 were investigated. Molecular typing was performed by APPCR. Depending on the number and size of amplified DNA bands, the strains were clustered into APPCR profiles.

Results: All strains of S. sonnei were typeable with this approach. APPCR generated nine indistinguishable bands ranged from 0.35 to 2.5 kbp in all studied strains. Only a single AP-PCR pattern was observed among the S. sonnei strains recovered in 2003. Two sporadic isolates recovered in 1984 showed different AP-PCR patterns compared to recent clinical isolates.

Conclusion: Results suggest that a very homogeneous AP-PCR cluster types might be responsible for shigellosis caused by S. sonnei in Tehran in 2003. Further molecular analysis conducted on a larger selection of isolates could confirm our findings.

Keywords

Acute diarrhea, Shigella sonnei, Molecular epidemiology, AP-PCR.

© 0, Archives of Clinical Infectious Diseases. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.

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