Antibiotic Susceptibility Pattern Among Staphylococcus spp. With Emphasis on Detection of mecA gene in Methicillin Resistant Staphylococcus aureus Isolates

Fateh Rahimi; Majid Bouzari; Zohreh Maleki; Fermisk Rahimi

Antibiotic Susceptibility Pattern Among Staphylococcus spp. With Emphasis on Detection of mecA gene in Methicillin Resistant Staphylococcus aureus Isolates

Fateh Rahimi ^{1
, *} , Majid Bouzari ¹ , Zohreh Maleki ² and Fermisk Rahimi ³

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¹ Department of Biology, Faculty of Science, University of Isfahan, Isfahan, IR Iran

² Department of Pathology, Tehran University of Medical Science, Imam Khomeini Hospital, Tehran, IR Iran

³ Department of Computer Engineering, Islamic Azad University

Article information

Archives of Clinical Infectious Diseases: 4 (3); 143-150
Article Type: Research Article

To Cite: Rahimi F, Bouzari M, Maleki Z, Rahimi F. Antibiotic Susceptibility Pattern Among Staphylococcus spp. With Emphasis on Detection of mecA gene in Methicillin Resistant Staphylococcus aureus Isolates, Arch Clin Infect Dis. Online ahead of Print ; 4(3):143-150.

Abstract

Background: Staphylococcus aureus is a major pathogen in hospital setting and in the community and causes a wide range of diseases. MRSA infection has recently become a serious problem in anti-microbial chemotherapy. The aim of the study was to detect and analyze the antibiotic diversity and isolation of methicillin resistance gene (mecA) of S. aureus isolated from Tehran hospitals as a rapid and reliable method.

Results: Totally, 321 (54.7%) isolates were identified as S. aureus. 66, 65, 88, 88, 100, 41, 38, 41, 0, 40, 93, 20 and 64% of S. aureus isolates were resistant to kanamycin, cephotaxim, methicillin, oxacillin, ampicillin, erythromycin, clindamycin, sulphamethoxazole-trimethoprime, vancomycin, chloramphenicol, ciprofloxacin, gentamicin and tetracycline, respectively. All MRSA and 63% of intermediate isolates carried mecA gene.

Conclusion: In contrary to other studies in Iran, the prevalence of methicillin resistance is rising up in Tehran and most of MRSA isolates were resistance to 5 antibiotics at least. Vancomycin, chloramphenicol, gentamicin and clindamycin are the most effective antibiotics. All MRSA isolates had mecA gene with different expression. Detection of mecA gene is a rapid and reliable method for identification of MRSA isolates.

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Antibiotic Susceptibility Pattern Among Staphylococcus spp. With Emphasis on Detection of mecA gene in Methicillin Resistant Staphylococcus aureus Isolates

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Archives of Clinical Infectious Diseases

Published by: Kowsar

Antibiotic Susceptibility Pattern Among Staphylococcus spp. With Emphasis on Detection of mecA gene in Methicillin Resistant Staphylococcus aureus Isolates

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