Archives of Clinical Infectious Diseases

Published by: Kowsar
Crossmark

Development and Validation of Heminested RT-PCR and qRT-PCR Assays for Comprehensive Detection of Rabies Virus in the Suspected Rabid Brain and Saliva Samples

Fereshteh Amiri 1 , Zohreh Fadajan 1 , Azadeh Rasooli 2 , Iman Salahshourifar 1 , Rouzbeh Bashar 3 , Masoumeh Arab Baferani 3 and Maryam Fazeli 3 , *
Authors Information
1 Department of Biology, College of Basic Sciences, Tehran Sciences and Research Branch, Islamic Azad University, Tehran, Iran
2 Department of Biochemistry, Faculty of Sciences, Payame-Noor University, Tehran, Iran
3 Virology Department, Pasteur Institute of Iran, Tehran, Iran
Article information
  • Archives of Clinical Infectious Diseases: 14 (2); e85790
  • Published Online: May 4, 2019
  • Article Type: Research Article
  • Received: October 27, 2018
  • Revised: February 2, 2019
  • Accepted: February 17, 2019
  • DOI: 10.5812/archcid.85790

To Cite: Amiri F , Fadajan Z , Rasooli A, Salahshourifar I , Bashar R , et al. Development and Validation of Heminested RT-PCR and qRT-PCR Assays for Comprehensive Detection of Rabies Virus in the Suspected Rabid Brain and Saliva Samples, Arch Clin Infect Dis. Online ahead of Print ; 14(2):e85790. doi: 10.5812/archcid.85790.

Abstract
Copyright © 2019, Archives of Clinical Infectious Diseases. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
1. Background
2. Objectives
3. Methods
4. Results
5. Discussion
Acknowledgements
Footnotes
References
  • 1. Albertini AA, Schoehn G, Weissenhorn W, Ruigrok RW. Structural aspects of rabies virus replication. Cell Mol Life Sci. 2008;65(2):282-94. doi: 10.1007/s00018-007-7298-1. [PubMed: 17938861].
  • 2. Leung AK, Davies HD, Hon KL. Rabies: Epidemiology, pathogenesis, and prophylaxis. Adv Ther. 2007;24(6):1340-7. doi: 10.1007/BF02877781. [PubMed: 18165217].
  • 3. Abera E, Assefa A, Belete S, Mekonen N. Review on rabies, with emphasis on disease control and eradication measures. Intl J Basic Appl Virol. 2015;4(2):60-70.
  • 4. Knobel DL, Cleaveland S, Coleman PG, Fevre EM, Meltzer MI, Miranda ME, et al. Re-evaluating the burden of rabies in Africa and Asia. Bull World Health Organ. 2005;83(5):360-8. [PubMed: 15976877]. [PubMed Central: PMC2626230].
  • 5. Singh R, Singh KP, Cherian S, Saminathan M, Kapoor S, Manjunatha Reddy GB, et al. Rabies - epidemiology, pathogenesis, public health concerns and advances in diagnosis and control: A comprehensive review. Vet Q. 2017;37(1):212-51. doi: 10.1080/01652176.2017.1343516. [PubMed: 28643547].
  • 6. Albertini AA, Ruigrok RW, Blondel D. Rabies virus transcription and replication. Adv Virus Res. 2011;79:1-22. doi: 10.1016/B978-0-12-387040-7.00001-9. [PubMed: 21601039].
  • 7. Dacheux L, Berthet N, Dissard G, Holmes EC, Delmas O, Larrous F, et al. Application of broad-spectrum resequencing microarray for genotyping rhabdoviruses. J Virol. 2010;84(18):9557-74. doi: 10.1128/JVI.00771-10. [PubMed: 20610710]. [PubMed Central: PMC2937603].
  • 8. Zhu S, Guo C. Rabies control and treatment: From prophylaxis to strategies with curative potential. Viruses. 2016;8(11). doi: 10.3390/v8110279. [PubMed: 27801824]. [PubMed Central: PMC5127009].
  • 9. Jackson AC. Human rabies: A 2016 Update. Curr Infect Dis Rep. 2016;18(11):38. doi: 10.1007/s11908-016-0540-y. [PubMed: 27730539].
  • 10. Lechenne M, Naissengar K, Lepelletier A, Alfaroukh IO, Bourhy H, Zinsstag J, et al. Validation of a rapid rabies diagnostic tool for field surveillance in developing countries. PLoS Negl Trop Dis. 2016;10(10). e0005010. doi: 10.1371/journal.pntd.0005010. [PubMed: 27706156]. [PubMed Central: PMC5051951].
  • 11. Madhusudana SN, Sukumaran SM. Antemortem diagnosis and prevention of human rabies. Ann Indian Acad Neurol. 2008;11(1):3-12. doi: 10.4103/0972-2327.40219. [PubMed: 19966972]. [PubMed Central: PMC2781142].
  • 12. Dacheux L, Reynes JM, Buchy P, Sivuth O, Diop BM, Rousset D, et al. A reliable diagnosis of human rabies based on analysis of skin biopsy specimens. Clin Infect Dis. 2008;47(11):1410-7. doi: 10.1086/592969. [PubMed: 18937576].
  • 13. Bourhy H, Cowley JA, Larrous F, Holmes EC, Walker PJ. Phylogenetic relationships among rhabdoviruses inferred using the L polymerase gene. J Gen Virol. 2005;86(Pt 10):2849-58. doi: 10.1099/vir.0.81128-0. [PubMed: 16186241].
  • 14. Dacheux L, Larrous F, Lavenir R, Lepelletier A, Faouzi A, Troupin C, et al. Dual combined real-time reverse transcription polymerase chain reaction assay for the diagnosis of lyssavirus infection. PLoS Negl Trop Dis. 2016;10(7). e0004812. doi: 10.1371/journal.pntd.0004812. [PubMed: 27380028]. [PubMed Central: PMC4933377].
  • 15. Picard-Meyer E, Peytavin de Garam C, Schereffer JL, Marchal C, Robardet E, Cliquet F. Cross-platform evaluation of commercial real-time SYBR green RT-PCR kits for sensitive and rapid detection of European bat Lyssavirus type 1. Biomed Res Int. 2015;2015:839518. doi: 10.1155/2015/839518. [PubMed: 25785274]. [PubMed Central: PMC4345247].
  • 16. Wacharapluesadee S, Hemachudha T. Ante- and post-mortem diagnosis of rabies using nucleic acid-amplification tests. Expert Rev Mol Diagn. 2010;10(2):207-18. doi: 10.1586/erm.09.85. [PubMed: 20214539].
  • 17. Heaton PR, Johnstone P, McElhinney LM, Cowley R, O'Sullivan E, Whitby JE. Heminested PCR assay for detection of six genotypes of rabies and rabies-related viruses. J Clin Microbiol. 1997;35(11):2762-6. [PubMed: 9350729]. [PubMed Central: PMC230057].
  • 18. David D, Yakobson B, Rotenberg D, Dveres N, Davidson I, Stram Y. Rabies virus detection by RT-PCR in decomposed naturally infected brains. Vet Microbiol. 2002;87(2):111-8. doi: 10.1016/S0378-1135(02)00041-X. [PubMed: 12034539].
  • 19. Echevarria JE, Avellon A, Juste J, Vera M, Ibanez C. Screening of active lyssavirus infection in wild bat populations by viral RNA detection on oropharyngeal swabs. J Clin Microbiol. 2001;39(10):3678-83. doi: 10.1128/JCM.39.10.3678-3683.2001. [PubMed: 11574590]. [PubMed Central: PMC88406].
  • 20. Orlowska A, Smreczak M, Trebas P, Zmudzinski JF. Comparison of real-time PCR and heminested RT-PCR methods in the detection of rabies virus infection in bats and terrestrial animals. Bull Vet Inst Pulawy. 2008;52(3):313-8.
  • 21. Logan JM, Edwards KJ, Saunders NA. Real-time PCR: Current technology and application. Horizon Scientific Press; 2009.
  • 22. Hoffmann B, Freuling CM, Wakeley PR, Rasmussen TB, Leech S, Fooks AR, et al. Improved safety for molecular diagnosis of classical rabies viruses by use of a TaqMan real-time reverse transcription-PCR "double check" strategy. J Clin Microbiol. 2010;48(11):3970-8. doi: 10.1128/JCM.00612-10. [PubMed: 20739489]. [PubMed Central: PMC3020878].

Featured Image:

Creative Commons License Except where otherwise noted, this work is licensed under Creative Commons Attribution Non Commercial 4.0 International License .

Search Relations:

Author(s):

Article(s):

Create Citiation Alert
via Google Reader

Readers' Comments