The VDR and TNF-? Gene Polymorphisms in Iranian Tuberculosis Patients: The Study on Host Susceptibility

AUTHORS

Mohammed Varahram 1 , Parissa Farnia 1 , * , Sabar Anoosheh 1 , Mehdi Kazampour 1 , Muayad Merza 1 , Shima Saeif 1 , Mohammad Reza Masjedi 1 , Ali Akbar Velayati 1

1 Mycobacteriology Research Centre, National Research Institute of Tuberculosis and Lung Disease (NRITLD), Shahid Beheshti University, M.C., Tehran, IR Iran

How to Cite: Varahram M, Farnia P, Anoosheh S, Kazampour M, Merza M, et al. The VDR and TNF-? Gene Polymorphisms in Iranian Tuberculosis Patients: The Study on Host Susceptibility, Arch Clin Infect Dis. Online ahead of Print ; 4(4):207-213.

ARTICLE INFORMATION

Archives of Clinical Infectious Diseases: 4 (4); 207-213
Article Type: Research Article

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Abstract

Background: VitaminD receptor (VDR) and tumor necrosis factoralpha (TNF-?) genes are thought to be important in the intracellular killing of mycobacteria. This study aimed to determine the association of VDR and TNF-? variant with development of pulmonary tuberculosis (PTB) among Iranian patients.

Patients and methods: Selected regions of VDR and TNF-? were amplified, and then the PCR products were digested using restriction enzyme (RFLP). Digested products were run on 8% polyacrylamide gel, and were stained with silver nitrate. Single nucleotide polymorphisms (SNPs) at restriction sites of BsmI, and FokI of VDR gene and SNPs of TNF-? at -238,-308, -244,-857,-863 positions were analyzed by PCR-RFLP among 117 PTB cases and 60 healthy controls.

Results: No statistically significant difference was observed in allele frequencies of FokI of VDR and TNF-? at -238, -244,-863 and 857 position. Although, the frequency of b allele of BsmI (p=0.001) and -308 A variant in TNF-? promoter region (p=0.006) were significantly more in PTB patients than healthy controls. The frequency of extended diplotypes were different in patients and control subjects (p<0.05).

Conclusion: This study confirmed the association of VDR BsmI and TNF-? -308A with susceptibility to tuberculosis in Iranian PTB patients. In addition, the results showed the importance of haplotypes and diplotypes analysis in determining the host susceptibility against TB

Keywords

VDR, TNF-? gene, Tuberculosis.

© 0, Archives of Clinical Infectious Diseases. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.

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