Comparison of the Value of Two Different Sputum Staining for Diagnosis of Acid Fast Bacilli

AUTHORS

Masood Ziaee 1 , * , Mohammad Namaei 1 , Majid Khazaei 2 , Ghodsieh Azarkar 1

1 Department of Microbiology, Birjand University of Medical Sciences, Birjand, IR Iran

2 Department of Physiology, Isfahan University of Medical Sciences, Isfahan, IR Iran

How to Cite: Ziaee M, Namaei M, Khazaei M, Azarkar G. Comparison of the Value of Two Different Sputum Staining for Diagnosis of Acid Fast Bacilli, Arch Clin Infect Dis. Online ahead of Print ; 3(2):99-102.

ARTICLE INFORMATION

Archives of Clinical Infectious Diseases: 3 (2); 99-102
Article Type: Brief Report

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Abstract

The aim of this study was to compare the diagnostic value of fluorochrome microscopy (FM) with Ziehl- Neelsen (ZN) staining in the diagnosis of tuberculosis (TB).

Patients and methods: In this study, 920 consecutive patients suspected of having pulmonary TB, referred to TB laboratory, provincial office of health care, Birjand University of Medical Sciences, recruited and a total of 2760 sputum specimens were collected from them. All samples were smeared and stained using both Ziehl Neelsen and auraminphenol methods as recommended by WHO. All positive smears by fluorescent microscopy were over-stained by ZN technique for confirmation. The sensitivity of ZN staining was also evaluated in different contamination conditions.

Results: A total of 102 out of 920 study subjects had pulmonary TB, of them 68 (66.66%) patients were smear positive by either staining method while others were smear negative. The proportion of positive smears detected was 51% and 57% for the ZN and auramine phenol staining methods, respectively. The sensitivity, specificity, positive predictive value and negative predictive value were 51%,100%,100%, 94% and 57%,100%,100,95% for the ZN and auramine phenol staining methods, respectively.

Conclusion: FM is more sensitive than ZN for diagnosis of TB. However, since FM is more sensitive and rapid, using this method in clinical laboratories with large specimen numbers is recommended.

Keywords

Mycobacterium tuberculosis, Ziehl Neelsen, Fluorescence microscopy

© 0, Archives of Clinical Infectious Diseases. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.

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