Rapid Diagnosis of Pulmonary Tuberculosis From Sputum by Polymerase Chain Reaction

AUTHORS

Mohammad Jobayer 1 , * , SM Shamsuzzaman 1 , Kazi Zulfiquer Mamun 1

1 Department of Microbiology, Dhaka Medical College, Dhaka, Bangladesh

How to Cite: Jobayer M, Shamsuzzaman S, Zulfiquer Mamun K. Rapid Diagnosis of Pulmonary Tuberculosis From Sputum by Polymerase Chain Reaction, Arch Clin Infect Dis. Online ahead of Print ; 9(2):20694. doi: 10.5812/archcid.20694.

ARTICLE INFORMATION

Archives of Clinical Infectious Diseases: 9 (2); 20694
Published Online: January 24, 2014
Article Type: Research Article
Received: September 15, 2013
Accepted: December 4, 2013
Crossmark

Crossmark

CHEKING

READ FULL TEXT
Abstract

Background: Tuberculosis (TB) is a curable infectious disease yet the leading cause of death worldwide and in Bangladesh it is responsible for 7% of total deaths every year.

Objectives: This cross sectional study was conducted to isolate and identify Mycobacterium tuberculosis from sputum and to evaluate the efficacy of polymerase chain reaction (PCR) for the early diagnosis of pulmonary tuberculosis.

Patients and Methods: A total of 150 clinically suspected pulmonary TB patients (male/female: 97/53; age: 31.9 9.8 years) were enrolled in this study. Freshly passed single morning sputum was collected from each patient and diagnostic efficacy of PCR was compared with staining and culture methods.

Results: Among 150 sputum samples, 25 (16.7%) were positive by Ziehl-Neelsen (ZN) stain, 37 (24.7%) yielded growth in Lowenstein-Jensen (LJ) media and 45 (30%) were positive by PCR. Of the 37 isolated Mycobacteria in culture, 36 were M. tuberculosis and one was identified as non-tuberculous Mycobacterium by PCR and biochemical tests. The mean detection time was 26.9 days for the culture and 12 hours for the PCR method. Considering the culture method as the gold standard, the sensitivity of PCR was 97.3% and specificity was 92% with accuracy of 93.3%. Twenty-one (16.8%) smear negative cases and nine (7.9%) culture negative samples were positive by the PCR method.

Conclusions: PCR was the most sensitive, accurate and rapid method for diagnosis of TB. It was concluded that, for the diagnosis of pulmonary tuberculosis, PCR directly from sputum may be recommended in Bangladesh, especially in suspected tuberculosis patients who remain negative by conventional methods.

Keywords

Bangladesh Mycobacterium tuberculosis Polymerase Chain Reaction Tuberculosis, Pulmonary

© 2014, Archives of Clinical Infectious Diseases. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.

Full Text

Full text is available in PDF

COMMENTS

LEAVE A COMMENT HERE: