Immunosuppressive Effect of Purified Pyocyanine Pigment on T-Lymphocytes Viability Against Experimental Infection with Hydatid Cyst Protoscolices

AUTHORS

Adel Talib Al Saeed 1 , * , Ahmed Mohammad Salih 2 , Zaman A A Ibrahim 3

1 College of Veterinary Medicine, University of Duhok, Duhok, Iraq

2 College of Nursing, University of Duhok, Duhok, Iraq

3 Department of Basic Sciences, College of Nursing, University of Baghdad, Iraq

How to Cite: Al Saeed A T, Mohammad Salih A, A A Ibrahim Z. Immunosuppressive Effect of Purified Pyocyanine Pigment on T-Lymphocytes Viability Against Experimental Infection with Hydatid Cyst Protoscolices, Arch Clin Infect Dis. Online ahead of Print ; 4(3):171-5.

ARTICLE INFORMATION

Archives of Clinical Infectious Diseases: 4 (3); 171-5
Article Type: Brief Report

Crossmark

CHEKING

READ FULL TEXT
Abstract

Background: The effect of pyocyanine pigment, which was isolated and purified from Pseudomonas aeruginosa, on specific lymphocytes viability inside the body of white male Balb/c mice against the experimental secondary hydatidosis and the infectivity of protoscolices was studied in comparison with negative control mice groups, phosphate buffered saline (PBS) and positive control group (immunoferon).

Materials and methods: Four groups of male Balb/c mice were intraperitoneally (IP) inoculated with four purified concentrations of pyocyanine (25, 50, 75, 100?m/ml). Seven days later, they were given the same concentrations as a booster dose of the pigment, then 7 days later they were intraperitoneally infected with 2000 protoscoleces /mL (PBS) as a challenge dose. The fifth group was intraperitoneally inoculated with 1ml of sterile PBS and used as a negative control group, while the sixth group was intraperitoneally inoculated with 100?mg/ml immunoferon and received the challenge dose of 2000 protoscoleces/ml PBS and served as the positive control group.

Results: The concentrations of 50, 75 and 100?m/ml of this pigment had suppressive effect on these specific immune response cells. This effect was statistically significant (p<0.01) after six weeks from the challenge dose with intraperitoneal protoscolices infection. This effect revealed that the protoscolices infectivity increased due to suppression viability of T lymphocytes, while the immunoferon showed a significant stimulation of these specific cellular cells, which decrease the protoscolices infectivity in comparison with higher pigment concentrations.

Conclusion: Pyocyanine is a toxic pigment causing suppression of T-cells activity, especially at higher concentrations which allow protoscolices development and growth.

Keywords

Pseudomonas aeruginosa, Pyocyanine, T cell, Protoscolices.

© 0, Archives of Clinical Infectious Diseases. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.

Full Text

Full text is available in PDF

COMMENTS

LEAVE A COMMENT HERE: