Saliva or Serum, Which is Better for the Diagnosis of Gastric Helicobacter Pylori Infection?

AUTHORS

Rasool Estakhri 1 , * , Homayoon Dolatkhah 2 , Ardavan Ghazanchaei 3 , Behrooz Pourasgari 4 , Manuchehr Nourazarian 4

1 Liver and Gastrointestinal Diseases Research Center, Tabriz University of Medical Sciences, Tabriz, IR Iran

2 Department of Biochemistry, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, IR Iran

3 Department of Immunology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, IR Iran

4 Clinical Laboratory, Imam Khomeini Educational Hospital, Tabriz University of Medical Sciences, Tabriz, IR Iran

How to Cite: Estakhri R, Dolatkhah H, Ghazanchaei A, Pourasgari B, Nourazarian M. Saliva or Serum, Which is Better for the Diagnosis of Gastric Helicobacter Pylori Infection?, Arch Clin Infect Dis. Online ahead of Print ; 3(3):121-5.

ARTICLE INFORMATION

Archives of Clinical Infectious Diseases: 3 (3); 121-5
Article Type: Research Article

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Abstract

Background: Helicobacter pylori is known as an agent which may involve in the occurrence of peptic ulcer, gastric cancer and also other known and unknown diseases. Treatment of the infection with antibiotics eradicates the disease and prevents its pathologic effects. A noninvasive and inexpensive method for detection of the infection is needed. In this study the diagnostic values of serum and saliva anti H. pylori IgG was evaluated.

Patients and methods: The saliva and blood samples were collected from 114 patients who underwent upper GI endoscopy and gastric biopsy. Tissue samples were examined by rapid urease test and microscopic study. Saliva and serum samples were tested by ELISA-based test for anti H. pylori IgG, using a commercial kit.

Results: From 114 cases, 61(53.5%) patients were positive for H. pylori in rapid urease test and microscopic study and 53(46.5%) were negative in both tests. Rates of positive result for H. pylori in patients with and without peptic ulcer were almost similar. Mean values of anti H. pylori IgG in saliva and serum of H. pylori positive patients were higher than H. pylori negative patients. Sensitivity, specificity, positive predictive value, negative predictive value and accuracy of tests in saliva were 83.6%, 71.7%, 77.3%, 79.1%, 78.1% and in serum were 90.2%, 86.8%, 88.7%, 88.4% and 88.6% respectively.

Conclusion: It was concluded that ELISA-based anti H. pylori IgG test in saliva could be used as an alternative diagnostic test in the absence of other invasive procedures.

Keywords

Anti H. pylori IgG, ELISA, Saliva

© 0, Archives of Clinical Infectious Diseases. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.

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