Production of haemophilus growth factor disks in Iran

AUTHORS

Saied Aminiafshar 1 , * , Masomeh Alimagham 1

1 Department of Infectious Disease and Tropical Medicine, Shaheed Beheshti University of Medical Sciences, Iran

How to Cite: Aminiafshar S, Alimagham M. Production of haemophilus growth factor disks in Iran, Arch Clin Infect Dis. Online ahead of Print ; 1(2):71-4.

ARTICLE INFORMATION

Archives of Clinical Infectious Diseases: 1 (2); 71-4
Article Type: Research Article

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Abstract

Background: Haemophilus influenza may cause severe infections in children and adults. The isolation and identification of Haemophilus spp. are not appropriately achieved in Iran, while numerous infections are ascribed to the different members of this genus. Lack of growth factors disks is our main shortage in this regard.

Materials and methods: We obtained haemin (X-factor) from human blood, and nicotinamidadenine di nucleotide (V factor) from yeast cells. The products were absorbed to the filter paper disks and a comparative analytical study was performed using prepared disks in comparison to commercial disks from Oxoid Company.

Results: Results revealed that the prepared disks were as useful, sensitive and potent as the Oxoid disks in isolation and identification of H. influenzae and H. parainfluenzae.

Conclusion: We have prepared growth factor disks according to the original formulations. They could be commercially produced and uses in microbiological laboratories.

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© 0, Archives of Clinical Infectious Diseases. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.

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